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A kind of composition and its preparation method and application

A technology of composition and proportion mixing, applied in application, drug combination, pharmaceutical formula, etc., can solve the problems such as food allergy curative effect needs to be improved, and achieve the effects of low toxicity, abundant resources, and simple extraction and separation process

Active Publication Date: 2020-11-10
CHANGCHUN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the technical defects of the prior art, and provides a composition and its preparation method and application, so as to solve the technical problem in the prior art that the curative effect of conventional drugs on food allergy needs to be improved

Method used

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  • A kind of composition and its preparation method and application
  • A kind of composition and its preparation method and application
  • A kind of composition and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A composition, the composition is composed of the following ingredients in parts by weight: 5 parts of isocurbisin, 3 parts of alizarin, and 2 parts of hydroxylizarin.

[0036] The composition is prepared by:

[0037] (1) Add 1 kg of madder root, add 8 L of 95% ethanol to heat and reflux extraction for 3 times, each time for 2 hours, filter, combine the filtrates, recover ethanol under reduced pressure, and dry to obtain 82.1 g of ethanol extract;

[0038] (2) Take 20 g of the ethanol extract, add 100 mL of ethyl acetate for ultrasonic extraction for 10 min, and filter to obtain the ethyl acetate extract;

[0039] (3) Repeat step (2), combine the ethyl acetate extracts, add 10% Na 2 CO 3 Solution 100mL extraction;

[0040] (4) Add concentrated hydrochloric acid to acidify Na 2 CO 3 The extract was left to stand and filtered to obtain 2.3 g of filter residue;

[0041] (5) Take 0.5g of the filter residue, dissolve it with 2 mL of chloroform-methanol (1:1), separate ...

Embodiment 2

[0046] Immunomodulatory effects in vitro:

[0047] Take U266 cells in good growth state, inoculate in 24-well plate, inoculate 1 mL per well (the number of cells is 2×10 5 ), add the composition prepared in Example 1 according to the concentration gradient, so that the final concentration is 0, 5, 10, 20, 40, 80 g / mL respectively, and placed in 5% CO 2 In the environment, after 6 days of incubation in a 37°C incubator, use an IgE kit to detect the IgE content in the culture solution in each well, and calculate the IC 50 The value was 9.64 g / mL. The above experimental results show that the composition provided in Example 1 can effectively inhibit the secretion of IgE by U266 cells in vitro.

Embodiment 3

[0049] Immunomodulatory effects in vivo in animal models of food allergy:

[0050]BALB / C mice (20±2g), 80 females, were randomly divided into 8 groups including A, B, C, D, E, F, G, and H. Group A was fed normally, and groups B-H were sensitized by intragastric administration of allergens (20 g mice each intragastric administration: ovalbumin 15 mg + cholera toxin adjuvant 200 μg), for 4 consecutive weeks, 3 times a week. On the 0th, 15th, 22nd, and 28th days, blood was collected from the mandibular venous plexus of the mice, 3-4 drops each time, and the IgE content in the serum was measured for IgE monitoring.

[0051] After the modeling was successful (the IgE in the mouse serum of the B-H group was more than 20 times higher than that of the A group), the B group was administered with normal saline for the control group, and the C and D groups were administered with high (1.5mg / kg) and low ( 3mg / kg) concentration of the embodiment 1 composition, E-H groups were fed with mad...

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Abstract

The invention provides a composition and its preparation method and application. The technical scheme uses madder root as raw material, extracts with ethyl acetate after ethanol reflux extraction, the extract is extracted with sodium carbonate solution, the extract is acidified and filtered to collect the filter residue, and then dissolved in chloroform-methanol with Sephadex LH 20 Separation to obtain madderin, isordizin, and hydroxyrubidin respectively. In the present invention, the rubikin, isorubidin and hydroxyrubidin are mixed in a weight ratio of 5:3:2 to obtain an antiallergic composition with exact ingredients. In vitro experiments show that the composition of the present invention can effectively inhibit the secretion of IgE from cells, thereby realizing the immune regulation effect; in vivo experiments prove that oral administration of the composition can exactly reduce the expression level of IgE, and then realize the anti-allergic effect, and the effect is comprehensively better than that of the natural extract of rubidin things and parties. The invention can be used to prepare food or medicine for treating food allergy, and compared with hormone medicine and antihistamine medicine, the invention has low toxicity and is safe and reliable.

Description

technical field [0001] The invention relates to the technical field of antiallergic drugs, further relates to the extraction technology of plant functional components, and specifically relates to a composition and its preparation method and application. Background technique [0002] Food allergy is an IgE-mediated and non-IgE-mediated immune response caused by a certain food or food additive, which in turn leads to an allergic reaction in the digestive system or systemically. In recent years, food allergies have become more frequent, and most of them are children. Due to the lack of effective desensitization therapy, in the prior art, the method of staying away from allergens is mainly used for treatment, which affects the quality of life of patients to a certain extent; the drugs that can effectively control food allergy symptoms are very limited, and most doctors use antihistamines Drugs used to relieve allergy symptoms. In addition to antihistamines in chemical drugs, m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/122A23L33/105A61P37/08
CPCA23L33/105A23V2002/00A61K31/122A61K36/74A61P37/08A61K2300/00A23V2200/304A23V2250/21
Inventor 陈玉娟焦洁朱文禹赵丽辉郝凤奇韩德明李伟葛淑敏
Owner CHANGCHUN UNIV OF SCI & TECH
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