Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method of all-natural enzyme fermentation black hair dye

A technology of black hair dye and natural enzyme, which is applied to medical preparations, pharmaceutical formulations, hair care and other directions containing active ingredients, can solve problems such as lack of pigment extraction technology, and achieve the effect of overcoming difficulties in extraction

Active Publication Date: 2021-06-15
九州益华(上海)生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Currently known plant pigment hair dyes that are available have achieved certain results, but there are still many problems. These products still cannot avoid adding harmful substances such as phenols and hydrogen peroxide in hair dyes, and lack mature pigments. Extraction technology

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of all-natural enzyme fermentation black hair dye
  • A kind of preparation method of all-natural enzyme fermentation black hair dye
  • A kind of preparation method of all-natural enzyme fermentation black hair dye

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1, using black soybeans to prepare tyrosinase liquid;

[0041] Mix black soybeans with a phosphate buffer solution with a pH of 7, the ratio of black soybeans to phosphate buffer solution is 10g:20ml, and then ultrasonically extract at 25°C for 2.5 hours to obtain an extract;

[0042] After centrifuging the extract at 3000r for 4min, take the supernatant, add ammonium sulfate with a mass concentration of 55% in the supernatant and let it stand for 3h, then continue to centrifuge at 4000r for 10min and pour off the supernatant, then add pH The phosphate buffer solution of 7 was shaken to obtain the tyrosinase solution;

[0043] Step 2, preparation of tyramide substrate solution

[0044] Dissolving tyramine in a phosphate buffer solution with a pH of 7 to prepare a tyramine substrate solution with a concentration of 0.01 g / mL;

[0045] Step 3, preparing the melanin precursor in the biological fermentation system

[0046] Mix the tyrosinase solution prepared in ste...

Embodiment 2

[0049] Step 1, using black soybeans to prepare tyrosinase liquid;

[0050] Mix black soybeans with phosphate buffer solution with a pH of 7.5, the ratio of black soybeans to phosphate buffer solution is 10g: 40ml, and then ultrasonically extract at 30°C for 3 hours to obtain an extract;

[0051] After centrifuging the extract at 3000r for 5min, take the supernatant, add ammonium sulfate with a mass concentration of 55% in the supernatant and let it stand for 3h, then continue to centrifuge at 4000r for 10min and pour off the supernatant, then add pH The phosphate buffer solution of 7.5 is shaken to obtain the tyrosinase solution;

[0052] Step 2, preparation of tyramide substrate solution

[0053] Dissolving tyramine in a phosphate buffer solution with a pH of 7.5 to prepare a tyramine substrate solution with a concentration of 0.03 g / mL;

[0054] Step 3, preparing the melanin precursor in the biological fermentation system

[0055] The tyrosinase solution prepared in step ...

Embodiment 3

[0058] Step 1, using black soybeans to prepare tyrosinase liquid;

[0059] Mix black soybeans with a phosphate buffer solution with a pH of 8, the ratio of black soybeans to phosphate buffer solution is 10g:50ml, and then ultrasonically extract at 40°C for 3.5h to obtain an extract;

[0060] After centrifuging the extract at 3000r for 6min, take the supernatant, add ammonium sulfate with a mass concentration of 55% to the supernatant and let it stand for 3h, then continue to centrifuge at 4000r for 10min and pour off the supernatant, then add pH 8 phosphate buffer shocks to obtain tyrosinase solution;

[0061] Step 2, preparation of tyramide substrate solution

[0062] Dissolving tyramine in a phosphate buffer solution with a pH of 8 to prepare a tyramine substrate solution with a concentration of 0.05 g / mL;

[0063] Step 3, preparing the melanin precursor in the biological fermentation system

[0064] The tyrosinase solution prepared in step 1 and the tyrosinase substrate so...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing an all-natural enzyme-fermented black hair dye. The steps are as follows: step 1, prepare tyrosinase liquid by using black soybeans; step 2, prepare tyramine substrate solution; step 3, prepare a biological fermentation system The melanin precursor; step 4, adding auxiliary agent and mordant to the melanin precursor prepared in step 3 to make a hair dye. The invention utilizes pure natural black soybeans and food-grade tyramine as raw materials, adopts a green biological fermentation method, and successfully prepares an all-natural black hair dye with blackening effect. It effectively overcomes the use of chemically synthesized hair dyes containing ammonia, hydrogen peroxide, p-phenylenediamine and its derivatives, which are harmful to the human body; overcomes the problem of difficult extraction of pigments in natural hair dyes, and is suitable for large-scale use in the market Scope promotion.

Description

technical field [0001] The invention belongs to the technical field of a method for preparing a hair dye, and in particular relates to a method for preparing an all-natural enzyme-fermented black hair dye. Background technique [0002] At present, most of the hair dyes sold on the market are chemical synthetic hair dyes. With the continuous improvement of people's attention to health, the carcinogenic and strong allergenic chemical dyeing ingredients (such as phenylenediamines, amino acids, etc.) contained in chemically synthesized hair dyes Phenol and other substances) and some harmful ingredients (such as hydrogen peroxide, ammonia, etc.), have attracted more and more attention. For this reason, people try to seek natural harmless, safer hair dyeing materials. [0003] Currently known plant pigment hair dyes that are available have achieved certain results, but there are still many problems. These products still cannot avoid adding harmful substances such as phenols and h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61K8/41A61Q5/10
CPCA61K8/415A61K2800/85A61Q5/10A61K8/9789
Inventor 杨谦孙文杏何仰清余晓皎姚秉华
Owner 九州益华(上海)生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com