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Method for preparing all-natural enzyme fermented black hair dye

A technology of black hair dye and natural enzyme, which can be applied to medical preparations containing active ingredients, hair care, pharmaceutical formulations, etc., can solve problems such as lack of pigment extraction technology, and achieve the effect of overcoming difficulties in extraction

Active Publication Date: 2019-01-25
九州益华(上海)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Currently known plant pigment hair dyes that are available have achieved certain results, but there are still many problems. These products still cannot avoid adding harmful substances such as phenols and hydrogen peroxide in hair dyes, and lack mature pigments. Extraction technology

Method used

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  • Method for preparing all-natural enzyme fermented black hair dye
  • Method for preparing all-natural enzyme fermented black hair dye
  • Method for preparing all-natural enzyme fermented black hair dye

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1, using black soybeans to prepare tyrosinase liquid;

[0041] Mix black soybeans with a phosphate buffer solution with a pH of 7, the ratio of black soybeans to phosphate buffer solution is 10g:20ml, and then ultrasonically extract at 25°C for 2.5 hours to obtain an extract;

[0042] After centrifuging the extract at 3000r for 4min, take the supernatant, add ammonium sulfate with a mass concentration of 55% in the supernatant and let it stand for 3h, then continue to centrifuge at 4000r for 10min and pour off the supernatant, then add pH The phosphate buffer solution of 7 was shaken to obtain the tyrosinase solution;

[0043] Step 2, preparation of tyramide substrate solution

[0044] Dissolving tyramine in a phosphate buffer solution with a pH of 7 to prepare a tyramine substrate solution with a concentration of 0.01 g / mL;

[0045] Step 3, preparing the melanin precursor in the biological fermentation system

[0046] Mix the tyrosinase solution prepared in ste...

Embodiment 2

[0049] Step 1, using black soybeans to prepare tyrosinase liquid;

[0050] Mix black soybeans with phosphate buffer solution with a pH of 7.5, the ratio of black soybeans to phosphate buffer solution is 10g: 40ml, and then ultrasonically extract at 30°C for 3 hours to obtain an extract;

[0051] After centrifuging the extract at 3000r for 5min, take the supernatant, add ammonium sulfate with a mass concentration of 55% in the supernatant and let it stand for 3h, then continue to centrifuge at 4000r for 10min and pour off the supernatant, then add pH The phosphate buffer solution of 7.5 is shaken to obtain the tyrosinase solution;

[0052] Step 2, preparation of tyramide substrate solution

[0053] Dissolving tyramine in a phosphate buffer solution with a pH of 7.5 to prepare a tyramine substrate solution with a concentration of 0.03 g / mL;

[0054] Step 3, preparing the melanin precursor in the biological fermentation system

[0055] The tyrosinase solution prepared in step ...

Embodiment 3

[0058] Step 1, using black soybeans to prepare tyrosinase liquid;

[0059] Mix black soybeans with a phosphate buffer solution with a pH of 8, the ratio of black soybeans to phosphate buffer solution is 10g:50ml, and then ultrasonically extract at 40°C for 3.5h to obtain an extract;

[0060] After centrifuging the extract at 3000r for 6min, take the supernatant, add ammonium sulfate with a mass concentration of 55% to the supernatant and let it stand for 3h, then continue to centrifuge at 4000r for 10min and pour off the supernatant, then add pH 8 phosphate buffer shocks to obtain tyrosinase solution;

[0061] Step 2, preparation of tyramide substrate solution

[0062] Dissolving tyramine in a phosphate buffer solution with a pH of 8 to prepare a tyramine substrate solution with a concentration of 0.05 g / mL;

[0063] Step 3, preparing the melanin precursor in the biological fermentation system

[0064] The tyrosinase solution prepared in step 1 and the tyrosinase substrate so...

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Abstract

The invention discloses a preparation method of an all-natural enzyme fermented black hair dye, which comprises the following steps: the step 1, preparing tyrosinase solution by black soybean; the step 2, preparing a tyramine substrate solution; the step 3, preparing melanin precursor in a biological fermentation system; and the step 4, adding auxiliary agent and mordant into the melanin precursorprepared in step 3 to prepare a hair dye. The invention utilizes pure natural black soybean and food-grade tyramine as raw materials, adopts green biological fermentation method, and successfully prepares all-natural black hair dye with black dyeing effect. The invention effectively overcomes the use of ammonia water, hydrogen peroxide, p-phenylenediamine and its derivatives in the chemically synthesized hair dye, which are harmful to human body. The invention overcomes the difficulty of extracting pigment from natural hair dye and is suitable for large-scale popularization in the market.

Description

technical field [0001] The invention belongs to the technical field of a method for preparing a hair dye, and in particular relates to a method for preparing an all-natural enzyme-fermented black hair dye. Background technique [0002] At present, most of the hair dyes sold on the market are chemical synthetic hair dyes. With the continuous improvement of people's attention to health, the carcinogenic and strong allergenic chemical dyeing ingredients (such as phenylenediamines, amino acids, etc.) contained in chemically synthesized hair dyes Phenol and other substances) and some harmful ingredients (such as hydrogen peroxide, ammonia, etc.), have attracted more and more attention. For this reason, people try to seek natural harmless, safer hair dyeing materials. [0003] Currently known plant pigment hair dyes that are available have achieved certain results, but there are still many problems. These products still cannot avoid adding harmful substances such as phenols and h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/41A61Q5/10
CPCA61K8/415A61K2800/85A61Q5/10A61K8/9789
Inventor 杨谦孙文杏何仰清余晓皎姚秉华
Owner 九州益华(上海)生物科技有限公司
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