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Application of rhodiola rosea and salidroside in the directed differentiation of stem cells into cardiomyocyte-like cells

A technique of cardiomyocyte-like cells and salidroside, applied in the field of Rhodiola, can solve the problems of low efficiency of induction and differentiation, long induction time and low function.

Active Publication Date: 2021-07-20
雷桅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The induction and differentiation conditions of each laboratory are different, the induction and differentiation mechanism is not yet clear, the induction and differentiation efficiency is low, the secretory ability of islets is only about 1% of normal islets, and the induction process is complicated, the induction time is long, the number of cells obtained is small, and the function is low
[0016] So far, there are no reports that rhodiola and salidroside can induce stem cells to differentiate into cardiomyocytes and repair myocardial damage.

Method used

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  • Application of rhodiola rosea and salidroside in the directed differentiation of stem cells into cardiomyocyte-like cells
  • Application of rhodiola rosea and salidroside in the directed differentiation of stem cells into cardiomyocyte-like cells
  • Application of rhodiola rosea and salidroside in the directed differentiation of stem cells into cardiomyocyte-like cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Reagents and materials

[0044]Salidroside (Sigma, USA), adipose-derived mesenchymal stem cells (ASCs) (Saiye Biotechnology Co., Ltd.), complete DMEM of adipose-derived mesenchymal stem cells (Saiye Biotechnology Co., Ltd.), osteogenic differentiation medium ( Saiye Biotechnology Co., Ltd.), adipogenic differentiation medium (Saiye Biotechnology Co., Ltd.), DMEM medium (Gibco, USA), double antibody mixture (Beijing Suo Laibao Company), fetal bovine serum (Hyclone, USA) company), rabbit anti-mouse cTnT polyclonal antibody (Cell Signaling Technology, USA), rabbit anti-mouse cTnI polyclonal antibody (Cell Signaling Technology, USA), rabbit anti-mouse α-actinin polyclonal antibody (Cell Signaling Technology, USA), rabbit Anti-mouse GATA-4 polyclonal antibody (U.S. Cell Signaling Technology Company).

[0045] Experimental steps:

[0046] (1) Adipose-derived mesenchymal stem cell culture

[0047] Rat adipose-derived mesenchymal stem cells were purchased from Biosciences In...

Embodiment 2

[0054] The reagents and materials of this embodiment are all the same as those in Example 1.

[0055] Experimental steps:

[0056] (1) Culture of adipose-derived mesenchymal stem cells: step (1) of this example is the same as that of Example 1.

[0057] (2) Hypoxia treatment induces directed differentiation of adipose-derived mesenchymal stem cells into cardiomyocyte-like cells

[0058] Take the ASCs of the third generation from the cultured place and inoculate them in a cell culture dish (low-sugar DMEM medium containing 2% fetal bovine serum, 100U / ml penicillin, 100U / ml streptomycin) at 37°C, 5% CO 2 The cells were cultured under certain conditions until the confluence rate of the cells was greater than 90%, and the cell culture medium was replaced with a basal medium, and three experimental groups were set up to culture under different hypoxic conditions.

[0059] Hypoxia induction group (Hypoxia, HC group): the oxygen content of three different induction groups were 2%, ...

Embodiment 3

[0062] The reagents and materials of this embodiment are all the same as those in Example 1.

[0063] Experimental steps:

[0064] (1) Culture of adipose-derived mesenchymal stem cells: step (1) of this example is the same as that of Example 1.

[0065] (2) Combined treatment of salidroside and hypoxia induces directed differentiation of adipose-derived mesenchymal stem cells into cardiomyocyte-like cells

[0066] Take the ASCs of the third generation from the cultured place and inoculate them in a cell culture dish (low-sugar DMEM medium containing 2% fetal bovine serum, 100U / ml penicillin, 100U / ml streptomycin) at 37°C, 5% CO 2 Cultivate under conditions until the cell confluency rate is greater than 90%, replace the cell culture medium with basal medium, and divide them into 3 experimental groups. The grouping and experimental conditions are as follows:

[0067] Salidroside+hypoxia treatment induction group (Hypoxia+SAL, HS group): This induction group has three different...

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Abstract

The invention provides the application of rhodiola rosea and salidroside in inducing the directed differentiation of stem cells into cardiomyocyte-like cells, especially the combination of salidroside and hypoxia as an inducer for inducing the directed differentiation of adipose-derived mesenchymal stem cells into myocardial-like cells like cells. The present invention develops a new application of salidroside, that is, inducing the directional differentiation of stem cells such as adipose stem cells into cardiomyocyte-like cells in vitro; provides the effect of rhodiola rosea and salidroside in inducing the directional differentiation of adult stem cells into cardiomyocyte-like cells, It is conducive to in-depth research in the field of in vivo stem cell transplantation in the treatment of acute and chronic myocardial injury and cardiovascular diseases, and provides a basis for the development of new drugs.

Description

technical field [0001] The invention relates to the application of rhodiola rosea, especially salidroside, in inducing directional differentiation of stem cells into cardiomyocyte-like cells. Background technique [0002] Rhodiola is a plant of the genus Rhodiola (Rhodiola L.) of the Crassulaceae family. It is a perennial herb or subshrub plant, mainly distributed in the Himalayas in the northern hemisphere, northwest Asia and North America at an altitude of 1600-4000 meters. area. Rhodiola rosea has a long history of medicinal use and is known as "golden ginseng". According to the Pharmacopoeia, Rhodiola is the dry root and stem root of Rhodiola grandiflora, a plant of the sedum family. It is sweet, bitter, and flat in nature. and burnout wheezing. Modern pharmacological studies have shown that the biological activity of rhodiola is inseparable from the various chemical components it contains. [0003] Salidroside (salidroside) is a natural product isolated and purified...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077A61K31/7032A61P9/10
CPCA61K31/7032A61P9/10C12N5/0657C12N2500/02C12N2500/30
Inventor 雷桅陈灿何原税晓容马睦棣
Owner 雷桅
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