Method for large-scale production of fetal stem cell secretin

A stem cell and secretin technology, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve problems such as a large number of difficulties in culturing, promote adherence and clone formation, avoid differentiation, and reduce costs and needs. effect of space
CN109295136AInactive Publication Date: 2019-02-01姬松茸科研基金有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
姬松茸科研基金有限公司
Publication Date
2019-02-01
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention discloses a method for large-scale production of mesenchymal stem cell secretin, comprising the following steps: 1) stem cells are cultured through a culture solution in a bioreactor, and the internal conditions of the bioreactor are 37 DEG C and 5 % carbon dioxide gas; 2) the stem cells obtained in the Step 1 are washed with a phosphate buffer in the bioreactor; then a cell culturemedium buffer is injected into the bioreactor to continuously culture the stem cells for 12 hours; and 3) a supernatant in the bioreactor is collected, and the supernatant undergoes ultracentrifugation to obtain a protein concentrate. According to the method for large-scale production of mesenchymal stem cell secretin, enzyme in cells and sulfhydryl groups in protein can be protected from being oxidized by adding beta-mercaptoethanol or monothioglycerol. With the addition of the leukemia inhibitory factor, the leukemia inhibitory factor can avoid stem cell differentiation and maintain stem cell pluripotency.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for large-scale production of fetal stem cell secretin. Background technique

[0002] Stem cells are a type of pluripotent cells with self-replicating ability. Under certain conditions, it can differentiate into a variety of functional cells. Stem cells are divided into embryonic stem cells and adult stem cells according to their developmental stage. Stem cells are divided into three categories according to their developmental potential: totipotent stem cells, pluripotent stem cells, and unipotent stem cells (multipotent stem cells). Stem cells are not fully differentiated and immature cells that have the potential to regenerate various tissues and organs and the human body. They are called "universal cells" in the medical field, and stem cells are the cells of origin. Stem cells are cells with proliferation and differentiation potential, have the ability of self-renewal ...

Claims

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