Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primers and method for identifying genetic polymorphism of Plasmodium vivax PvMSP-3alpha and application of primers

A technology of Plasmodium and α gene, which is applied in the field of primers for identifying the polymorphism of Plasmodium vivax PvMSP-3α gene, can solve problems such as failure to develop a vaccine, and achieve the effect of single band and strong specificity

Inactive Publication Date: 2019-02-01
STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Despite decades of research into malaria vaccines, vaccines that are effective for field use against malaria infection have yet to be developed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primers and method for identifying genetic polymorphism of Plasmodium vivax PvMSP-3alpha and application of primers
  • Primers and method for identifying genetic polymorphism of Plasmodium vivax PvMSP-3alpha and application of primers
  • Primers and method for identifying genetic polymorphism of Plasmodium vivax PvMSP-3alpha and application of primers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Identification of PvMSP-3α gene polymorphism of Plasmodium vivax

[0030] 1 material

[0031] 1.1 Genomic DNA of Plasmodium vivax

[0032] Blood filter papers from patients infected with Plasmodium vivax were collected from malaria endemic areas in China-Myanmarborder (CMB). Use a hole punch to cut the blood filter paper into small pieces with a diameter of about 3cm, 3 pieces, put them into a 1.5ml centrifuge tube, and extract the samples to be tested according to the operation manual of the QIAamp DNA Blood Mini Kit. Genomic DNA of Plasmodium vivax.

[0033] 1.2 Main reagents

[0034]

[0035] 2 method

[0036] 2.1 Amplification of the PvMSP-3α gene of Plasmodium vivax

[0037] The fragment was amplified according to the PvMSP-3α gene of the standard strain of Plasmodium vivax, and 3 pairs of specific primers were designed using the primer design software of Shanghai Yingjun Biotechnology Co., Ltd. The specific primers were synthesized by Shanghai Yingjun Biotechnolog...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses primers and method for identifying genetic polymorphism of Plasmodium vivax PvMSP-3alpha. The primers include an upstream primer (shown as SEQ ID NO: 1) and a downward primer (shown as SEQ ID NO: 2). The specific primers are designed according to regional characteristics of genetic polymorphism; a DNA fragment containing a conserved region and a polymorphic region is acquired by PCR (polymerase chain reaction); whether PvMSP-3alpha gene mutates can be identified through sequencing and analysis; the gene type being wild or mutant can be distinguished then. In addition, the invention also discloses application of the specific primers for identifying genetic polymorphism of Plasmodium vivax PvMSP-3alpha in the preparation of detection tools for identifying genetic polymorphism of Plasmodium vivax PvMSP-3alpha.

Description

Technical field [0001] The invention belongs to the field of biological detection, and specifically relates to primers and methods for identifying the PvMSP-3α gene polymorphism of Plasmodium vivax. Background technique [0002] Malaria is an important parasitic disease that seriously endangers human health and affects social and economic development. According to statistics from the World Health Organization (WHO), malaria was endemic in 91 countries and regions around the world in 2016, with 216 million cases, an increase from 2015 5 million and the number of deaths was 445,000. In 2016, my country reported a total of 3321 cases of malaria. [0003] With the efforts of governments and international organizations around the world, from 2000 to 2016, the number of malaria cases has decreased by 18%, and the number of deaths has decreased by 47%. However, in recent years, in Myanmar, Laos, Cambodia, Thailand, and Vietnam in Southeast Asia, there have been reports of malaria parasi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6893C12N15/11
CPCC12Q1/6893C12Q2600/156Y02A50/30
Inventor 陈绅波陈军虎王越沈海默崔延冰徐斌
Owner STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com