Primer, detection method and kit for detecting human EGFR (Epidermal Growth Factor Receptor) gene T790M mutation

Abstract

The invention provides a primer, detection method and kit for detecting human EGFR (Epidermal Growth Factor Receptor) gene T790M mutation. The detection primer provided by the invention can be combined with an EGFR gene conserved sequence to amplify a mutation sequence. The detection method provided by the invention can be combined with an amplified fragment by adopting a fluorescence signal detection group to send out a fluorescence signal; a blocking agent is specifically combined with a wild type sequence corresponding to a mutation site to inhibit wild type non-specific amplification. A competitive allele specific PCR (Polymerase Chain Reaction) amplification technology is adopted to establish the kit based on real-time fluorescence PCR and the EGFR gene T790M mutation can be accurately detected. The method provided by the invention has the advantages of simplicity in operation, easiness of reading results and high sensitivity, and can be used for detecting a sample containing 0.001 percent of the EGFR gene T790M mutation; ultrasensitive detection of the EGFR gene T790M mutation is realized.

Description

[0001] This application claims the priority of the Chinese patent application submitted to the China Patent Office on October 13, 2017, the application number is 201710955204.4, and the invention title is "Primers, detection methods and kits for ultrasensitive detection of human EGFR gene mutations" , the entire contents of which are incorporated in this application by reference. technical field [0002] The invention belongs to the field of biotechnology, and in particular relates to a primer, a detection method and a kit for detecting T790M mutation of human EGFR gene. Background technique [0003] Human epidermal growth factor receptor (EGFR) is a protein tyrosine kinase receptor widely distributed on the surface of mammalian epithelial cells, fibroblasts, glial cells, keratinocytes and other cells, with tyrosine Acid kinase activity. It is a member of the HER / ErbB family, so it is also known as HER1 or ErbB1. After EGFR binds to its ligand, it forms a dimer on the cell...

AI Technical Summary

Problems solved by technology

The high-resolution melting curve method uses special instruments, which are difficult to popularize in hospitals, and this method is less effective in detecting deletion mutations

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