Hybridoma cell strain secreting anti-serine phosphorylated tau protein monoclonal antibody
A hybridoma cell line, serine phosphorylation technology, applied in the field of immunodetection, can solve the problems of less research and other issues
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[0019] Example 1: Anti-human p-Tau 396,404 Preparation of specific monoclonal antibodies
[0020] For the convenience of reference below, the meanings of the main abbreviations are explained as follows:
[0021] p-Tau 396,404 Monomer, i.e., p-Tau 396,404 Monomer: A single protein formed from human Tau protein phosphorylated at serine 396 and 404.
[0022] p-Tau 396,404 aggregates, i.e., p-Tau 396,404 Oligomer: Human Tau protein phosphorylated at serine 396 and 404 is obtained by mutual folding.
[0023] 1. Antigen preparation
[0024] The Tau protein (p-Tau 396,404 ), dissolved in TBS solution to a final concentration of 1 mg / mL. Result: The results of SDS-PAGE showed that the synthetic peptide was of good purity and the electrophoresis bands were uniform ( figure 1 in A).
[0025] 2. Preparation of mouse monoclonal antibody
[0026] 2.1. Immunization of mice
[0027] Mix 100 μL of the prepared antigen with an equal volume of Freund’s complete adjuvant for the firs...
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