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A kind of ssr molecular marker bvre105 for detecting graininess of beet seeds and its application

A molecular marker and related molecular marker technology, which can be used in the determination/inspection of microorganisms, DNA/RNA fragments, biochemical equipment and methods, etc. To achieve the effect of reducing workload and shortening cycle

Active Publication Date: 2021-06-29
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve the problem that the existing molecular markers related to sugar beet seed graininess are lacking, and it is not easy to identify the graininess of seeds, and provides a SSR molecular marker BvRE105 for detecting graininess of sugar beet seeds and its application

Method used

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  • A kind of ssr molecular marker bvre105 for detecting graininess of beet seeds and its application
  • A kind of ssr molecular marker bvre105 for detecting graininess of beet seeds and its application
  • A kind of ssr molecular marker bvre105 for detecting graininess of beet seeds and its application

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specific Embodiment approach 1

[0026] Specific embodiment one: the present embodiment detects the SSR molecular marker BvRE105 of sugar beet seed grain property, and the primer pair that is used for PCR amplification this molecular marker is BvRE105S and BvRE105A, and specific sequence is:

[0027] BvRE105S: 5'-TCACCAGGATTAACCAAGCC-3'

[0028] BvRE105 A: 5'-ACTTCCACGTCTTTGCAACC-3'.

[0029] The molecular marker of this embodiment can be used to judge the seed quality of sugar beet germplasm or individuals. When in use, directly take the young leaves of annual plants or other young tissues for DNA extraction and marker detection, and do not need to spend two years on sowing-mother root cultivation-mother root planting-biennial plant traits investigation; biennial plants are also preferred. The young leaves, young inflorescences or other young tissues of the plants are subjected to DNA extraction and marker detection. When used to judge the graininess of the entire germplasm, it can be judged directly based...

specific Embodiment approach 2

[0030] Embodiment 2: The application of the SSR molecular marker BvRE105 for detecting graininess of sugar beet seeds in this embodiment is used to detect graininess of seeds.

specific Embodiment approach 3

[0031] Specific embodiment three: the difference between this embodiment and specific embodiment two is: the specific detection method is:

[0032] 1. Separation and extraction of total DNA from young sugar beet tissues;

[0033] Two, using the DNA obtained in step 1 as a template, using primers BvRE105 S and BvRE105 A to carry out PCR amplification,

[0034] 3. The PCR amplification product obtained in step 2 is separated by polyacrylamide gel electrophoresis, and the result is determined according to the size of the amplification product. If only a band of 219bp is detected, it is determined to be a sugar beet seed single grain; if 207bp is detected The bands of , 231bp and 279bp were determined as multiple sugar beet seeds. Others are the same as in the second embodiment.

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Abstract

An SSR molecular marker BvRE105 for detecting graininess of beet seeds and application thereof, belongs to the technical field of molecular markers, and relates to an SSR molecular marker for detecting graininess of sugarbeet seeds and its application. The purpose of the method is to solve the problem that the existing molecular markers related to sugar beet seed graininess are not easy to be identified. The primer pair used for PCR amplification of the molecular marker is BvRE105 S and BvRE105 A. The molecular marker BvRE0105 of the present invention has a consistency of 70% between the marker detection results and the performance of seed grain traits in all individuals, and 86.87% and 53.33% in single-grain and multi-seed individuals respectively. The invention is used for the particle property detection of sugar beet seeds.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and relates to an SSR molecular marker for detecting grain properties of sugar beet seeds and an application thereof. Background technique [0002] The fruit of sugar beet (Beta Vulgaris) is surrounded by persistent lignified calyx, forming a special "bulb" structure: when the flower is solitary, the bulb is a single achene, and when multiple sessile florets are aggregated to form In a cyme, multiple fruits in the bulb are closely connected to form a conglomerate containing multiple achenes. When describing sugar beet germplasm resources, the "seed grain" trait is used to describe them, and they are divided into single seed (also known as monoembryonic) and polyzymotic (also known as polyembryonic). [0003] The emergence rate of multi-seed species is high, which is beneficial to seedling preservation, while single-seed species is conducive to mechanized cultivation and saves the workl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6869C12N15/11
CPCC12Q1/6869C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈丽王希赵春雷
Owner HEILONGJIANG UNIV