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Dopamine and dopamine metabolite based molecularly imprinted magnetic nanospheres as well as preparation method and application thereof

A magnetic nanosphere and molecular imprinting technology, applied in chemical instruments and methods, alkali metal compounds, and other chemical processes, to achieve rapid and high-selectivity separation and enrichment, good water solubility, and improved selective recognition capabilities

Active Publication Date: 2019-02-15
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Dopamine and dopamine metabolite based molecularly imprinted magnetic nanospheres as well as preparation method and application thereof
  • Dopamine and dopamine metabolite based molecularly imprinted magnetic nanospheres as well as preparation method and application thereof
  • Dopamine and dopamine metabolite based molecularly imprinted magnetic nanospheres as well as preparation method and application thereof

Examples

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Embodiment 1

[0035] A method for preparing dopamine and its metabolite molecularly imprinted magnetic nanospheres, comprising the following steps:

[0036] 1) Put 0.30g of ferric chloride, 1.00g of sodium acetate, 40mg of sodium polyacrylate, 10mL of diethylene glycol and 5mL of ethylene glycol in a reaction kettle, and react at 160°C for 5h. After the reaction, wash the reaction product with ultrapure water to neutral, and vacuum-dried to obtain carboxyl-functionalized magnetic nanospheres;

[0037] The vacuum drying temperature is 40° C., the pressure is 0.04 MPa, and the drying time is 4 hours.

[0038] 2) Add 150 mg of carboxyl-functionalized magnetic nanospheres obtained in step 1) and two adrenaline metabolites to 30 mL of phosphate buffer to obtain a dual template-carrier complex;

[0039] The two epinephrine metabolites are DA and DOPAC, the dosage of DA is 20 mg, and the dosage of DOPAC is 30 mg; the phosphate buffer is a mixed solution of sodium dihydrogen phosphate and disodium h...

Embodiment 2

[0056] A method for preparing dopamine and its metabolite molecularly imprinted magnetic nanospheres, comprising the following steps:

[0057] 1) Put 0.40g of ferric chloride, 1.50g of sodium acetate, 50mg of sodium polyacrylate, 12mL of diethylene glycol and 6mL of ethylene glycol in a reaction kettle, and react at 180°C for 6h. After the reaction, use ultrapure water to dissolve the reaction The product was washed until neutral, and then vacuum-dried to obtain carboxyl-functionalized magnetic nanospheres; the vacuum drying temperature was 47° C., the pressure was 0.06 MPa, and the drying time was 5 hours.

[0058] 2) Add 300 mg of carboxyl-functionalized magnetic nanospheres obtained in step 1) and two adrenaline metabolites to 50 mL of phosphate buffer to obtain a dual template-carrier complex;

[0059] The two epinephrine metabolites are DA and DOPAC, and the dosages of the two are 30 mg and 20 mg respectively; the phosphate buffer is an aqueous solution of sodium dihydrog...

Embodiment 3

[0075] A method for preparing dopamine and its metabolite molecularly imprinted magnetic nanospheres, comprising the following steps:

[0076] 1) Put 0.50g of ferric chloride, 2.50g of sodium acetate, 80mg of sodium polyacrylate, 20mL of diethylene glycol and 10mL of ethylene glycol in a reaction kettle, and react at 200°C for 10 hours. After the reaction, use ultrapure water to dissolve the reaction The product was washed until neutral, and then vacuum-dried to obtain carboxyl-functionalized magnetic nanospheres; the vacuum drying temperature was 60° C., the pressure was 0.08 MPa, and the drying time was 7 hours.

[0077] 2) Add 200 mg of carboxyl-functionalized magnetic nanospheres obtained in step 1) and two adrenaline metabolites to 40 mL of phosphate buffer to obtain a dual template-carrier complex;

[0078] The two epinephrine metabolites are DA and DOPAC, both of which are used in an amount of 30 mg; the phosphate buffer is a mixed solution of sodium dihydrogen phosphat...

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Abstract

The invention discloses dopamine and dopamine metabolite based molecularly imprinted magnetic nanospheres as well as a preparation method and an application thereof. The method comprises the followingsteps: synthesizing carboxyl-functionalized magnetic nanospheres with a hydrothermal method; adding the carboxyl-functionalized magnetic nanospheres and two epinephrine metabolites to a phosphate buffer solution in a certain ratio to obtain a dual-template-carrier complex; stirring a separation product, functional monomer hyperbranched polyethyleneimine and crosslinking agent glutaraldehyde in the phosphate buffer solution for polymerization to obtain a polymer; separating a solid polymer out by externally applying a magnetic field; finally, performing reflux elution on the solid polymer, andperforming vacuum drying to obtain the dopamine and dopamine metabolite based molecularly imprinted magnetic nanospheres. The preparation method is simple, conditions are mild, and the prepared molecularly imprinted magnetic nanospheres are uniform in particle size, high in stability and good in water solubility, and can realize selective enrichment and detection of dopamine and 3,4-dihydroxyphenylacetic acid in complicated matrix of human fluid simultaneously and rapidly.

Description

technical field [0001] The invention relates to the technical fields of molecular recognition, magnetic separation, nanomaterials and neuroscience, and in particular to a molecularly imprinted magnetic nanosphere of dopamine and its metabolites, a preparation method and application thereof. Background technique [0002] Dopamine (DA) is a key monoamine neurotransmitter in the central nervous system, which is closely related to the occurrence and development of various neurodegenerative and affective disorders. In Parkinson's disease (PD), changes in the levels of DA and its metabolite 3,4-dihydroxy-phenylacetic acid (DOPAC) can be used as sensitive indicators for the early diagnosis of PD. At the same time, levodopa (DA precursor substance) is widely used clinically as one of the main therapeutic drugs for PD patients. However, with the long-term use of levodopa, PD patients often have treatment side effects such as dyskinesia, and the severity of dyskinesia Correlation wit...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01J20/30C08L33/02C08L79/02C08K5/07C08J3/24
CPCB01J20/268B01J20/28007B01J20/28009B01J20/28019C08J3/246C08J2333/02C08J2479/02C08K5/07
Inventor 高瑞霞赵雅韵闫庆封芳杨梦瑶张军杰
Owner XI AN JIAOTONG UNIV
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