Micro-fluidic chip for screening rare cells in whole blood

Abstract

The invention discloses a micro-fluidic chip for screening rare cells in whole blood. The micro-fluidic chip comprises a microchannel as well as a platelet removing region formed on the microchannel by a first interdigital electrode assembly, a red cell lysis region formed by a lysis pipeline segment on the microchannel, a red cell removing region formed on the microchannel by a second interdigital electrode assembly, a white cell removing region formed on the microchannel by a magnetic field and a target cell extraction region formed on the microchannel through a third interdigital electrodeassembly in sequence. According to the micro-fluidic chip disclosed by the invention, platelets and lysed red cells are removed by using the size difference; white cells are removed by using a specific monoclonal antibody and a magnetic bead mode, and screening purity and capture ratio of target cells are favorably improved; in addition, the micro-fluidic chip can clean samples. The system disclosed by the invention does no harm to cells, can be used for conventional flow cell sorting and screening the rare cells and has important significance for promoting noninvasive prenatal screening and tumor prognosis detection.

Description

technical field [0001] The invention relates to the technical field of microfluidic chip, biological particle detection and manipulation, in particular to a microfluidic chip for screening rare cells in whole blood. Background technique [0002] The detection captures the tiny rare cells in the blood, which is helpful for the early diagnosis of diseases and the monitoring of patients' conditions. The existing flow cytometry sorter has the problems of large volume, complex structure, repeated cleaning of the pipeline, and time-consuming labor; and the sorting process is completed in the air, and the system is open, which will generate air containing samples such as cells, bacteria, and viruses. Sol contamination limits its clinical application. At present, most of the cell sorting systems of companies such as BD and Beckman Coulter use the Jet-in-Air electrostatic deflection separation method (US Patent No. 3710933, No. 3826364). Although cells can be separated at high speed...

AI Technical Summary

Problems solved by technology

[0003] Existing microfluidic cell sorting schemes, such as electroosmotic flow, electrophoresis, pneumatic control, mechanical valves, optical tweezers, and photothermogel, have the problems of slow sorting speed, complex structure, and high cost; Throughput cell sorting methods such as membrane filtration, dielectrophoresis, ultrasound, and surface acoustic wave separation methods depend on the characteristics of the cells themselves, and their universality is poor.

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