Dopamine detection device, detection electrode and preparation method as well as electrochemical sensor

Abstract

The invention designs two functional molecules according to the characteristics of a dopamine molecule. The dopamine molecule has two characteristic groups including o-diphenolhydroxyl and fatty primary amine at the same time and the two functional molecules are used for specifically identifying o-diphenolhydroxyl and fatty primary amine groups on dopamine respectively, and the selectivity of a dopamine electrochemical sensor can be remarkably improved. The invention provides a dopamine electrochemical sensing technology based on double-molecule identification, and a traditional non-identification or single identification strategy is broken to realize major breakthrough of a dopamine sensor; the double-molecule identification can be finished in one liquid drop and the capacity of a neededdopamine sample is greatly reduced; high-sensitivity detection of dopamine in a trace volume can be realized, and the double-molecule identification has very important practical significance and application prospect.

Description

technical field [0001] The invention relates to a method for detecting dopamine, in particular to a method for detecting dopamine based on bimolecular recognition, a detection electrode and a preparation method thereof, and an electrochemical sensor for detecting dopamine; it belongs to the technical field of biological detection. Background technique [0002] Dopamine is an important neurotransmitter in the central nervous system in vivo, and plays a key role in regulating brain function. Its dysregulation is related to a variety of brain neurodegeneration. Nervous system diseases such as depression, schizophrenia, Parkinson's disease, etc. are closely related to abnormal levels of dopamine. Therefore, measuring the dynamic changes of dopamine in the living brain is technically of great significance for in-depth understanding of the role of dopamine in normal and abnormal brain functions. [0003] There are two ways to detect the level of dopamine in the living brain. One ...

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Problems solved by technology

[0005] In view of the prior art, the detection of dopamine in the brain is easily interfered by a variety of electrical activity interfering substances, and the present invention solves the selectivity problem of sensor detection of dopamine

Since the volume of living brain samples is extremely small, as low as μL level, and the concentration of dopamine in the brain is as low as nM level, dilution makes common sensors fail to meet the requirements of sensitive detection. The present invention solves the problem of sensors detecting μL level volume samples

Aiming at the problems of poor selectivity, low sensitivity, and large sample volume required for the detection of dopamine in the current electrochemical sensor, the present invention proposes a scheme for detecting dopamine, and designs a highly sensitive and selective detection method for dopamine. Electrode and electrochemical biosensor for dopamine detection in microscale biological samples

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