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Application of an effective inhibitor of type III phosphatidylinositol phosphokinase in the preparation of medicines for treating or preventing influenza virus infection

A technology for virus infection and prevention of influenza, applied in the field of medicine, can solve problems such as undiscovered and achieve broad-spectrum anti-influenza virus activity

Active Publication Date: 2021-10-22
浙江安维珞诊断技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no information about 6-amino-N-[3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3,2-d]pyrimidine Related reports of -2-yl]phenyl]-3-pyridinecarboxamide against influenza virus

Method used

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  • Application of an effective inhibitor of type III phosphatidylinositol phosphokinase in the preparation of medicines for treating or preventing influenza virus infection
  • Application of an effective inhibitor of type III phosphatidylinositol phosphokinase in the preparation of medicines for treating or preventing influenza virus infection
  • Application of an effective inhibitor of type III phosphatidylinositol phosphokinase in the preparation of medicines for treating or preventing influenza virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: 6-amino-N-[3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3,2-d]pyrimidin-2-yl Evaluation of the anti-influenza virus activity of ]phenyl]-3-pyridinecarboxamide

[0029] 1. Experimental materials

[0030] 1.1 Cells, viruses and drugs

[0031] MDCK cells were purchased from ATCC, and the virus A / PuertoRico / 8 / 34 (H1N1) was amplified from chicken embryo culture, 6-amino-N-[3-[4-(4-morpholino)pyrido[3', 2':4,5]furo[3,2-d]pyrimidin-2-yl]phenyl]-3-pyridinecarboxamide (CAS: 371942-69-7) was purchased from Sigma Company.

[0032] 1.2 Experimental Instruments

[0033] Multifunctional detector PerkinElmer, inverted microscope Costar.

[0034] 2. Experimental methods and results

[0035] 2.1 Cell culture

[0036] 37°C, 5% CO 2 cultured in a humidified incubator. A DMEM medium containing 10% FBS, 100 U / mL of penicillin and streptomycin was used. Cells were subcultured to 90% confluence, and the subculture ratio was 1 / 3–1 / 4.

[0037] 2.2 Virus culture

[0038] Take ...

Embodiment 2

[0049] Example 2: 6-amino-N-[3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3,2-d]pyrimidin-2-yl Evaluation of broad-spectrum anti-influenza virus of ]phenyl]-3-pyridinecarboxamide

[0050] On the basis of Example 1, this example further detects 6-amino-N-[3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3 ,2-d]pyrimidin-2-yl]phenyl]-3-pyridinecarboxamide to influenza A virus subtype H1N1 amantadine-resistant strains, influenza A virus subtype H6N6 strains, influenza A virus subtype Antiviral activity of H7N8 strain and influenza B virus.

[0051] 1. The virus strains used in the experiment include: A / WSN / S31N (H1N1), A / Duck / Hubei / 5 / 2010 (H6N6), A / Duck / Hubei / 216 / 1983 (H7N8), B / human / Huber / 1 / 2007

[0052] 2. Divide MDCK cells into 1.5×10 4 Cells / well were seeded in 96-well cell culture plates, cultured in a 37°C cell culture incubator for 14-18 hours, and the cells were grown into a single layer before use. The culture medium in the well plate was discarded, washed twice with PBS, 5....

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Abstract

The invention discloses the application of an effective inhibitor of type III phosphatidylinositol phosphokinase in the preparation of medicines for treating or preventing influenza virus infection. The research result of the present invention proves: a kind of effective inhibitor of type III phosphatidylinositol phosphokinase, i.e. small molecule compound 6-amino-N-[3-[4-(4-morpholino)pyrido[3' ,2':4,5]furo[3,2-d]pyrimidin-2-yl]phenyl]-3-pyridine carboxamide can dose-dependently inhibit influenza A virus subtype H1N1 adamantine in the non-toxic range Alkylamine-resistant strains, influenza A virus subtype H6N6 strains, influenza A virus subtype H7N8 strains and replication of influenza B viruses have very broad-spectrum anti-influenza virus activity. Therefore, a safe, highly effective small molecular compound with low toxicity and side effects is provided for the clinical treatment of influenza.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to an effective inhibitor of type III phosphatidylinositol phosphokinase 6-amino-N-[3-[4-(4-morpholino)pyrido[3',2' : Application of 4,5]furo[3,2-d]pyrimidin-2-yl]phenyl]-3-pyridinecarboxamide in the preparation of medicines for treating or preventing influenza virus infection. Background technique [0002] Influenza viruses belong to the family Orthomyxoviridae, the genus Influenzavirus. According to the differences in the antigenic and genetic characteristics of the virion nucleoprotein (NP) and matrix protein (M), influenza viruses are divided into three types: A, B, and C, also known as A, B, and C. The whole genome of influenza A virus consists of eight single-stranded negative-strand RNAs of different sizes, named after segment 1 to segment 8, respectively. The full length of the viral genome is about 13.6kb, encoding 10 structural proteins (PB2, PB1, PA, HA, NP, NA, M1, M2...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/519A61P31/16
CPCA61K31/519A61P31/16
Inventor 邬开朗刘芳祝成亮张艳朱莹刘兴晖
Owner 浙江安维珞诊断技术有限公司
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