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Application of dicoumarin in preparation of medicine for treating or preventing influenza virus infection

A technology of dicoumarin and influenza prevention, applied in the field of medicine, can solve the problems such as no reports that dicoumarin is anti-influenza virus and treat diseases caused by influenza virus infection, and achieves broad-spectrum anti-influenza virus activity and safety. good effect

Inactive Publication Date: 2015-10-21
武汉威立得生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the anti-influenza virus and treatment of influenza virus infection caused by dicoumarol

Method used

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  • Application of dicoumarin in preparation of medicine for treating or preventing influenza virus infection
  • Application of dicoumarin in preparation of medicine for treating or preventing influenza virus infection
  • Application of dicoumarin in preparation of medicine for treating or preventing influenza virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Evaluation of anti-influenza virus activity of dicoumarin

[0032] 1. Experimental Materials

[0033] 1.1 Cells, viruses and drugs

[0034] MDCK cells were purchased from American Type Culture Collection (ATCC); virus strain: A / PuertoRico / 8 / 34 (H1N1); drug: dicoumarin was purchased from Sigma.

[0035] 1.2 Experimental Instruments

[0036] Multilabel microplate reader (Perkin Elmer).

[0037] 2. Experimental methods and results

[0038] 2.1 Cell culture: 37°C, 5% CO 2 cultured in a humidified incubator. A DMEM medium containing 10% FBS, 100 U / mL of penicillin and streptomycin was used. Cells were subcultured after reaching 90% confluence, and the subculture ratio was 1 / 3 – 1 / 4.

[0039] 2.2 Virus culture: Take SPF chicken embryos aged 9 to 11 days, check them with an egg tester before inoculating the virus, and mark them at a position far away from the embryos, disinfect and punch holes, and inoculate 2- 4 The virus liquid with hemagglut...

Embodiment 2

[0049] Example 2: Evaluation of the broad-spectrum anti-influenza virus effect of dicoumarin

[0050] In this experiment, dicoumarin was tested against influenza A subtype H1N1 strains, influenza A virus subtype H3N2 strains, influenza A virus subtype H1N1 amantadine-resistant strains, influenza A virus subtype H6N6 strains, and influenza A virus subtype H6N6 strains. Antiviral activity of influenza virus subtype H7N8 strain, influenza A virus subtype H9N2 strain and influenza B virus.

[0051] 1. The virus strains used in the experiment include: A / Human / Hubei / 1 / 2009(H1N1), A / human / Hubei / 3 / 2005(H3N2), A / human / WSN / 33 / (H1N1,S31N), A / Duck / Hubei / 5 / 2010(H6N6), A / Duck / Hubei / 216 / 1983(H7N8), A / Chicken / Jiangsu / 1 / 2005(H9N2), B / human / Hubei / 1 / 2007.

[0052] 2. Divide MDCK cells into 1.5×10 4 Cells / well were seeded in 96-well cell culture plates, cultured in a 37°C cell culture incubator for 14-18 hours, and the cells were grown into a single layer before use. Discard the medium in...

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PUM

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Abstract

The invention discloses an application of dicoumarol to the preparation of a drug for treating or preventing influenza virus infection. Firstly, the toxicity effect of an MDCK (Madin Darby Canine Kidney) cell on the dicoumarol is detected, and the result shows that the maximum non-toxic consistency of the dicoumarol is 50.0 mum; secondly, the antivirus activity of the MDCK cell is detected within a completely non-toxic consistency range, and the result shows that the small molecular compound has remarkable antivirus activity, and the antiviral effect has a dosage dependence effect; finally, the antiviral activity of the dicoumarol for resisting different types and subtypes of influenza viruses is detected, and the result shows that the dicoumarol can resist the copy of a detected influenza virus strain in a dosage independence manner and the influenza virus resistance activity is broad-spectrum. Therefore, the dicoumarol disclosed by the invention is a novel broad-spectrum influenza virus resistant drug, and can be applied to the preparation of drugs for preventing or treating the influenza virus infection.

Description

technical field [0001] The invention relates to the application of dicoumarin in the preparation of medicines for treating or preventing influenza virus infection, and belongs to the technical field of medicine. Background technique [0002] Influenza viruses belong to the family Orthomyxoviridae, the genus Influenzavirus. According to the differences in the antigenic and genetic characteristics of the virion nucleoprotein (NP) and matrix protein (M), influenza viruses are divided into three types: A, B, and C, also known as A, B, and C. The whole genome of influenza A virus consists of eight single-stranded negative-strand RNAs of different sizes, named after segment 1 to segment 8, respectively. The full-length viral genome is about 13.6 kb, encoding 10 structural proteins (PB2, PB1, PA, HA, NP, NA, M1, M2, PB1-F2 and NS2 / NEP) and non-structural proteins (NS1). Influenza A viruses can be further divided into 17 H (H1-H17) and 10 N (N1-N10) subtypes according to the diff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/37A61P31/16
Inventor 陈绪林安利伟
Owner 武汉威立得生物医药有限公司
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