Abelmoschus esculentus L. seed total flavone extracting process parameter optimizing method and extracting method
A technology of okra seeds and extraction process, which is applied in the directions of multi-objective optimization, design optimization/simulation, pharmaceutical formulation, etc., can solve the problems of not being able to clarify the flavonoid content of okra seeds, not considering the interaction of experimental factors, and long extraction time, etc. Accurate and reliable parameters, solving multivariate problems, and the effect of short extraction time
Inactive Publication Date: 2019-03-12
四川省植物工程研究院
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Problems solved by technology
Both of the above two patents use okra fruit as the raw material, and the content of flavonoids in okra seeds cannot be clarified, and the extraction time is relatively long
In addition, both patents adopt single-factor experimental design, which only considers the influence of a single variable on the results, and does not consider the interaction between experimental factors, which often leads to systematic errors
In addition, in the prior art, the leaching time of okra total flavonoids is more than 30 minutes, which takes too long and consumes a lot of energy.
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[0049] A method for optimizing extraction process parameters of total flavonoids from okra seeds based on response surface methodology includes the following processes:
[0050] (1) Collect the tender fruits of okra, remove the pods, collect the seeds, dry them in a 40°C oven to a constant weight, and pass through 100 meshes for use after crushing.
[0051] (2) Preparation of rutin standard solution and formulation of standard curve
[0052] Accurately weigh 15.00mg of the standard rutin, dissolve it with methanol (chromatographically pure) and dilute to a 100mL volumetric flask to obtain a standard solution of rutin with a concentration of 0.15mg / mL. Using high-performance liquid method, Agilent G1329B autosampler draws 0.2μL, 0.4μL, 1.0μL, 3.0μL, 5.0μL, and analyzes according to the high-performance liquid method.
[0053] The standard curve drawn is like figure 1 , Rutin standard product and sample chromatogram such as figure 2 . by figure 1 It can be seen that the standard quali...
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The invention discloses an abelmoschus esculentus L. seed total flavone extracting process parameter optimizing method and an extracting method. The method is characterized in that influences of factors, such as alcohol concentration, a solid-liquid ratio, leaching time, temperature and the like, on total flavone extracting are comprehensively considered, and extracting conditions are optimized through a response surface model, so that a novel extracting process is obtained, and therefore, total flavone of abelmoschus esculentus L. seeds can be efficiently and accurately extracted. Compared with the prior art, the extracting process disclosed by the invention is scientific and reasonable, is short in extracting time, is high in total flavone extracting rate, and is low in cost.
Description
Technical field [0001] The invention belongs to the technical field of natural extracts, and specifically relates to a method for optimizing the extraction process parameters of total flavonoids of okra seeds based on response surface methodology and a method for extracting okra flavonoids. Background technique [0002] Okra (Abelmoschus esculentus L.) belongs to the Malvaceae (Malvaceae) Abelmoschus Medic. It is an annual herb and a vegetable and medicinal plant. The staple food part of okra is tender fruit, which is rich in nutrients such as protein, vitamins, carotene, sugar, etc., as well as various biologically active ingredients such as flavonoids and free amino acids. Flavonoids have the functions of eliminating active oxygen free radicals, anti-oxidation, anti-cancer, anti-tumor, anti-inflammatory, anti-allergic, protecting liver, and protecting the cardiovascular and cerebrovascular system. Therefore, the content of okra flavonoids can be used as Evaluation index of int...
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IPC IPC(8): A61K36/185G06F17/50
CPCA61K36/185A61K2236/333G06F30/20G06F2111/06
Inventor 张景荣刘玉珊叶昌华杨马进卓明李臻张军孙婷许震寰
Owner 四川省植物工程研究院