Method for optimizing extraction process parameter of total flavonoids from okra see and extraction method thereof
A technology for extraction process of okra seeds, which is applied in multi-objective optimization, design optimization/simulation, pharmaceutical formulations, etc., can solve the problems that the content of flavonoids in okra seeds cannot be determined, the interaction of experimental factors is not considered, and the extraction time is long. Accurate and reliable parameters, solving multivariate problems, and the effect of short extraction time
Inactive Publication Date: 2019-01-25
四川省植物工程研究院
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Problems solved by technology
Both of the above two patents use okra fruit as the raw material, and the content of flavonoids in okra seeds cannot be clarified, and the extraction time is relatively long
In addition, both patents adopt single-factor experimental design, which only considers the influence of a single variable on the results, and does not consider the interaction between experimental factors, which often leads to systematic errors
In addition, in the prior art, the leaching time of okra total flavonoids is more than 30 minutes, which takes too long and consumes a lot of energy.
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[0047] A method for optimizing the extraction process parameters of total flavonoids from okra seeds based on response surface methodology comprises the following processes:
[0048] (1) Collect tender okra fruits, remove the pods, collect the seeds, dry them in an oven at 40°C to constant weight, crush them and pass them through 100 meshes for later use.
[0049] (2) Preparation of rutin standard solution and establishment of standard curve
[0050] Accurately weigh 150.00 mg of rutin standard substance, dissolve it with methanol (chromatographically pure) and dilute it to a 100 mL volumetric flask, and its concentration is 1.5 mg / mL rutin standard solution. Using high performance liquid chromatography, Agilent G1329B autosampler draws 0.2 µL, 0.4 µL, 1.0 µL, 3.0 µL, 5.0 µL, and analyzes according to the high performance liquid phase method.
[0051] The standard curve drawn as figure 1 , rutin standard and sample chromatograms are shown in figure 2 . Depend on figure...
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A method for optimizing extraction process parameter of total flavonoids from okra see and its extracting method are disclosed, this method considered the influence of ethanol concentration, ratio ofmaterial to solvent, extraction time and temperature on the extraction of total flavonoids, and optimized the extraction conditions by response surface model to obtain a new extraction process, whichcan extract the total flavonoids of okra seeds efficiently and accurately. Compared with the existing process, the extraction process of the invention is scientific and reasonable, the extraction timeis short, the extraction rate of total flavonoids is high, and the cost is low.
Description
technical field [0001] The invention belongs to the technical field of natural extracts, and specifically relates to a method for optimizing extraction process parameters of total flavonoids from okra seeds based on a response surface method and an extraction method of flavonoids from okra seeds. Background technique [0002] Okra ( Abelmoschus esculentus L.) belongs to Malvaceae ( Malvaceae ) Okra ( Abelmoschus Medic ), an annual herb, a vegetable and medicinal plant. The staple food part of okra is tender fruit, which is rich in protein, vitamins, carotene, sugar and other nutrients, as well as flavonoids, free amino acids and other bioactive components. Flavonoids have medicinal and health effects on the human body, such as eliminating active oxygen free radicals, anti-oxidation, anti-cancer, anti-tumor, anti-inflammatory, anti-allergic, protecting the liver, and protecting the cardiovascular and cerebrovascular systems. Therefore, the content of flavonoids in okra ...
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IPC IPC(8): A61K36/185G06F17/50
CPCA61K36/185A61K2236/333G06F30/20G06F2111/06
Inventor 张景荣刘玉珊叶昌华杨马进卓明李臻张军孙婷许震寰
Owner 四川省植物工程研究院