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A mesoporous polydopamine-encapsulated liquid fluorocarbon ultrasound contrast agent and its preparation method

A technology of mesoporous polydopamine and ultrasound contrast agent, which is applied in the field of biomedical materials, can solve the problem of incapability of liquid fluorocarbon encapsulation, etc., and achieves the effects of stable in vivo and in vitro properties, good biocompatibility, and good ultrasound contrast effect.

Active Publication Date: 2021-06-04
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, polydopamine nanospheres are a kind of solid nanoparticles, which cannot contain liquid fluorocarbons as the inner core to form nanoscale ultrasound contrast agents.

Method used

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  • A mesoporous polydopamine-encapsulated liquid fluorocarbon ultrasound contrast agent and its preparation method
  • A mesoporous polydopamine-encapsulated liquid fluorocarbon ultrasound contrast agent and its preparation method
  • A mesoporous polydopamine-encapsulated liquid fluorocarbon ultrasound contrast agent and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 MPDA ultrasound contrast agent with a particle size of 82nm

[0035] Step 1: Dissolve 0.03g of dopamine hydrochloride and 0.1g of F-127 in 5mL of ethanol and 5mL of deionized water, and stir for 30min. Under ultrasonic conditions in a water bath, 160 μL of TMB was added dropwise, and milky white micelles were formed after shaking for 10 minutes. Then, under the condition of electromagnetic stirring, 160 μL of ammonia water was added dropwise. After stirring at room temperature for 4 h, the solution turned black. The solution was collected and centrifuged in EP tube for 10min (13000rmp). Wash three times with ethanol and water respectively, and finally resuspend in deionized water to prepare mesoporous polydopamine (MPDA) solution.

[0036]Step 2: Dry the above MPDA solution into a solid, weigh 10 mg and place it in a 25 mL round bottom flask. After vacuuming for 3 minutes, quickly inject 100 μL of perfluoropentane, shake well for 3 minutes, sonicate in a wa...

Embodiment 2

[0037] Example 2 MPDA ultrasound contrast agent with a particle size of 200nm

[0038] (1) Same as Step 1 of Example 1, except that the amount of dopamine hydrochloride is 0.15 g.

[0039] (2) Same as Step 2 of Example 1, except that 100 μL of perfluorohexane was quickly injected to prepare MPDA ultrasound contrast agent.

[0040] Measure its particle size with Malvern (Malvern) laser particle size analyzer to be 275nm, and obtain dynamic light scattering particle size distribution columnar image 3 , the particle size distribution range is narrow, and the particle size is relatively uniform. by TEM figure 2 It can be seen that the particle size is 200 nm. The particle size of MPDA ultrasound contrast agent can be controlled by changing the amount of dopamine hydrochloride.

Embodiment 3

[0041] Example 3 In Vitro Ultrasonic Imaging Test of MPDA Ultrasonic Contrast Agents at Different Mechanical Indexes and Different Frequencies

[0042] Take the MPDA ultrasonic contrast agent prepared in Example 1 above and add it to a dropper filled with degassed water, place it in a water tank with a temperature of 37°C, and use a Doppler ultrasonic diagnostic instrument probe to perform a mechanical index (MI ) were 0.13, 0.33 and the ultrasonic imaging experiment with frequencies of 6.5MHz and 8MHz respectively. Depend on Figure 4 It can be seen that the MPDA ultrasound contrast agent has obvious contrast effects under different mechanical index and different frequency conditions, and the contrast effect is better under high frequency and high mechanical index conditions. This shows that the MPDA ultrasound contrast agent after ultrasonic irradiation produces a large number of gas microbubbles, which makes the nano-particles realize the "liquid-gas" transformation, and t...

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Abstract

The invention discloses an ultrasonic contrast agent containing liquid fluorocarbon in mesoporous polydopamine and a preparation method thereof. Mesoporous polydopamine solutions (MPDA ultrasound contrast agents) with different particle sizes loaded with liquid fluorocarbons were prepared by template method using dopamine hydrochloride, block copolymer F-127 and 1,3,5-trimethylbenzene as basic raw materials . The mesoporous polydopamine (MPDA) prepared by the invention has excellent biocompatibility and mesoporous structure, and liquid fluorocarbons can enter into the mesoporous channels, and under heating and ultrasonic irradiation, bubbles are generated to enhance the ultrasound contrast effect. The particle size of the ultrasonic contrast agent is controllable, and the preparation method is simple. Co-packaging with drugs is expected to be further applied to the integration of tumor diagnosis and treatment, and has high research value and broad application prospects.

Description

Technical field: [0001] The invention belongs to the field of biomedical materials, and in particular relates to an ultrasonic contrast agent and a preparation method thereof. Background technique: [0002] Ultrasound imaging is an imaging technique that uses the physical characteristics of ultrasound and the differences in the acoustic properties of human organs and tissues to diagnose diseases. Ultrasound contrast agents were first used in ultrasound diagnosis, which can effectively improve the contrast quality of ultrasound images, enhance two-dimensional ultrasound images and Doppler signals of blood flow in solid organs such as muscles, liver, kidneys, and brain, and significantly improve the effect of ultrasound on the shape and quality of lesion areas. Type discrimination ability, enhance the sensitivity and specificity of ultrasound diagnosis. [0003] At present, the ultrasound contrast agent used clinically in my country is Sonovue, which is an ultrasound microbub...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/22A61K47/34A61K41/00
CPCA61K41/0052A61K47/34A61K49/223
Inventor 曹众朱瑾瑾
Owner SUN YAT SEN UNIV
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