Rotator cuff bioremediation mesh patch as well as use and preparation method thereof

A rotator cuff and biological technology, applied in the field of rotator cuff biological repair mesh and its use and preparation, can solve the problems of tissue regeneration mismatch, cytotoxicity, slow degradation rate, etc., to reduce the incidence of inflammation and infection, and accelerate Effect of functional reconstruction, good mechanical properties

Active Publication Date: 2019-03-12
上海白衣缘生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Due to the lack of mechanical strength of the acellular material patch in practical application, chemical cross-linking agents such as epoxy or glutaraldehyde need to be introduced in the process of processing, or used together with synthetic materials, which has the following disadvantages: Potential cell Toxicity, slow degradation rate, mismatch with tissue regeneration, can lead to fibrosis, chronic inflammation and other reactions

Method used

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  • Rotator cuff bioremediation mesh patch as well as use and preparation method thereof
  • Rotator cuff bioremediation mesh patch as well as use and preparation method thereof
  • Rotator cuff bioremediation mesh patch as well as use and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Preparation of Porcine Small Intestinal Submucosal Rotator Cuff Bioprosthetic Mesh, see Figure 5 :

[0057] (1) Pretreatment

[0058] Freshly slaughtered porcine small intestine tissues were cleaned, soaked in 0.5% acetic acid solution for 30 minutes, the ratio of porcine small intestine to acetic acid solution was 1:5, and the mucosal layer, muscular layer and serosa layer of porcine small intestine and jejunum were removed by physical scraping , lymph nodes, the submucosa was separated, cut into thin strips evenly in the longitudinal direction, and washed 3 times with purified water to obtain the rotator cuff biological repair material, that is, the small intestinal submucosa, hereinafter referred to as SIS material.

[0059] (2) Virus inactivation

[0060] Use a mixed aqueous solution containing 1.0% peracetic acid and 15% ethanol, the ratio of the SIS material to the mixed aqueous solution is 1:10, and soak at room temperature for 100 minutes under ultrasonic con...

Embodiment 2

[0071] Preparation of bio-mesh for rotator cuff repair in porcine small intestine submucosa

[0072] (1) Pretreatment

[0073] Freshly slaughtered pig small intestine tissues were cleaned and soaked in 0.01% acetic acid solution for 120 minutes. The ratio of pig small intestine to acetic acid solution was 1:10. The mucosal layer, muscular layer and serosa layer of pig small intestine jejunum were removed by physical scraping , Lymph nodes, the submucosa was separated, cut into pieces, and washed 3 times with purified water.

[0074] (2) Virus inactivation

[0075] Use a mixed aqueous solution containing 0.5% peracetic acid and 25% ethanol, the ratio of the SIS material to the mixed aqueous solution is 1:15, and soak at room temperature for 120 minutes under ultrasonic conditions to inactivate the virus. Afterwards, it was ultrasonically washed 3 times with purified water.

[0076] (3) Degreasing

[0077] Use ethanol with a concentration of 90%, the ratio of SIS material to...

Embodiment 3

[0086] For the safety of the samples, the samples prepared in Example 1-2 were tested for immunogenic substances.

[0087] (1) Detection method of residual cells: fixed with 10% neutral formalin, embedded in paraffin, cut into thin slices of 0.4 micron, dewaxed with xylene, dehydrated with serial alcohol, stained with hematoxylin-eosin, and microscope Next, observe the cell residue and matrix fiber structure.

[0088] (2) Detection method of DNA content: according to YY / T 0606.25-2014 "Method for determination of DNA residues in biological materials of animal origin: fluorescent staining method".

[0089] (3) Detection method of α-Gal antigen content: After the sample was fixed with paraformaldehyde, it was routinely embedded in paraffin and sectioned, with a thickness of 3 μm. Immunohistochemical reaction was carried out by using the specific affinity between biotin-labeled BSI-B4 and α-Gal antigen. Judgment of staining results: dark brown-yellow particles are strongly posi...

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Abstract

The invention discloses a rotator cuff bioremediation mesh patch as well as use and a preparation method thereof. The rotator cuff bioremediation mesh patch is prepared by taking animal tissues as a raw material, cells, DNA and other immune components are removed, the raw material is twisted into threads after being cut into thin strips, and threads are woven into the patch. A heterogeneous decellularized material obtains greater strength by being twisted into the threads, thus being less likely to break; furthermore, after weaving, due to the interlacing between the threads, the external force of stretching tear does not act on one point but on multiple interlacing points; therefore, the patch has good mechanical properties, and overcomes the disadvantages of poor mechanical strength of the rotator cuff bioremediation mesh patch prepared from the heterogeneous decellularized material and the need to introduce a cross-linking agent or a synthetic material; a mesh structure is obtainedby using a weaving process, and the mesh aperture can be adjusted by adjusting weaving parameters, so that the actual application demands can be met.

Description

technical field [0001] The invention relates to the field of biomaterials, in particular to a rotator cuff bio-repair mesh and its use and preparation method. Background technique [0002] The rotator cuff is the main anatomical structure that maintains the stability of the shoulder joint. Rotator cuff injury is a common disease, which is common in young adults and the elderly. It is caused by traumatic injury or degeneration of the rotator cuff tendon due to abnormal anatomical structure. For large rotator cuff tears, common treatments include conservative management, subacromial decompression, long head biceps tenectomy, partial cuff repair, latissimus dorsi tendon transfer, and repair with mesh or other materials Surgery and reverse total shoulder arthroplasty. These treatments have different indications and postoperative effects vary greatly. According to the analysis, surgical mesh repair can reduce pain, achieve higher satisfaction, and be more conducive to the reco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/58
CPCA61L27/3633A61L27/3687A61L27/50A61L27/58A61L2430/40
Inventor 赵金忠蒋佳韩韦红葛翠兰钱锵周秀清
Owner 上海白衣缘生物工程有限公司
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