Application of primer combination in identifying species of cattle and/or identifying beef
A primer combination and beef technology, which is applied in the application field of species identification and/or beef identification, can solve the problems of potential safety hazards, high requirements for operators, and inability to effectively distinguish buffalo, yellow cattle and yak LAMP primer combinations, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0265] The identification of embodiment 1 yellow beef
[0266] 1. Preparation of primers
[0267] The primer sequences used were synthesized by Shenggong Company. Six primers were designed for the target sequence, including two inner primers (FIP and BIP), two outer primers (F3 and B3) and two loop primers (LF and LB), the nucleotide sequences of which are listed in Table 1.
[0268] The specific configuration of the LAMP detection system is 0.12 μL each of 0.3 mM outer primers F3 and B3; 0.96 μL each of 2.4 mM inner primers FIP and BIP; 0.4 μL each of 1 mM loop primers LF and LB; 10 μL of 2× reaction buffer; 1 μL For sample DNA, add sterilized purified water to 20 μL system.
[0269] The reaction condition of the LAMP detection is: constant temperature at 65° C. for 50 minutes.
[0270] Table 1. Primer information used in the loop-mediated isothermal amplification technique
[0271]
[0272] 2. Preparation of nucleic acid DNA template
[0273] About 50 mg of fresh mus...
Embodiment 2
[0291] The identification of embodiment 2 buffalo meat
[0292] 1. Preparation of Primers
[0293] The primer sequences used were synthesized by Shenggong Company. Six primers were designed for the target sequence, including two inner primers (FIP and BIP), two outer primers (F3 and B3) and two loop primers (LF and LB), the nucleotide sequences of which are listed in Table 3.
[0294] The specific configuration of the LAMP detection system is 0.12 μL each of 0.3 mM outer primers F3 and B3; 0.96 μL each of 2.4 mM inner primers FIP and BIP; 0.4 μL each of 1 mM loop primers LF and LB; 10 μL of 2× reaction buffer; 1 μL For sample DNA, add sterilized purified water to 20 μL system.
[0295] The reaction condition of the LAMP detection is: constant temperature at 65° C. for 50 minutes.
[0296] Table 3. Primer information used in the loop-mediated isothermal amplification technique
[0297]
[0298] 2. Preparation of Nucleic Acid DNA Template
[0299] About 50 mg of fresh mu...
Embodiment 3
[0317] The identification of embodiment 3 yak meat
[0318] 1. Preparation of Primers
[0319] The primer sequences used were synthesized by Shenggong Company. Six primers were designed for the target sequence, including two inner primers (FIP and BIP), two outer primers (F3 and B3) and two loop primers (LF and LB), the nucleotide sequences of which are listed in Table 5.
[0320] The specific configuration of the LAMP detection system is 0.12 μL each of 0.3 mM outer primers F3 and B3; 0.96 μL each of 2.4 mM inner primers FIP and BIP; 0.4 μL each of 1 mM loop primers LF and LB; 10 μL of 2× reaction buffer; 1 μL For sample DNA, add sterilized purified water to 20 μL system.
[0321] The reaction condition of the LAMP detection is: constant temperature at 65° C. for 50 minutes.
[0322] Table 5. Primer information used in the loop-mediated isothermal amplification technique
[0323]
[0324] 2. Preparation of Nucleic Acid DNA Template
[0325] About 50 mg of fresh muscle...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Sensitivity | aaaaa | aaaaa |
| Sensitivity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More