Application of primer combination in duck species identification and/or duck meat identification

Abstract

The invention relates to the technical field of biology, in particular to application of a primer combination in duck species identification and / or duck meat identification. The primer combination ishigh in specificity and can accurately identify a duck or duck meat in a sample. An LAMP technology is used for detecting duck nucleic acid DNA in meat-based samples such as raw meat and meat processed foods in real time in a real-time fluorescent PCR machine. The screened primer has high sensitivity and high specificity, an established detection method has the advantages of high accuracy, short detection time and real-time observation of results, only 1 hour is needed from sample processing to reporting the results, the operation is simple, higher specificity is achieved compared with other PCR methods, and a higher detection speed is achieved than existing methods. The LAMP primer for detecting chicken meat can detect 20 fg of nucleic acid within 50 min of the total reaction time, a fluorescent method LAMP is used for detecting, the results can be observed in real time, and the risk of pollution is avoided.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to the application of a primer combination in duck species identification and / or duck meat identification. Background technique [0002] Meat is the main source of high-quality protein and various nutrients for human beings, and it is rich in essential amino acids and various trace elements. In recent years, with the improvement of the living standard of Chinese residents, the meat produced only in China is far from meeting the market demand, and some meat needs to be imported from abroad. These factors have created conditions for illegal traders at home and abroad to take advantage of the opportunity, and the adulteration and fraud of meat in the market have also attracted more and more attention. Although duck meat is a kind of meat that is very healthy for the body, at the same time, duck meat is often used as adulterated meat to imitate expensive meat such as mutton and bee...

AI Technical Summary

Problems solved by technology

However, the general PCR reaction takes at least 1.5 hours, and later detection requires gene sequencing and gel electrophoresis, and EB in gel electrophoresis is highly carcinogenic and has certain safety hazards

However, real-time fluorescent PCR requires expensive instruments and reagents, and the reaction time is at least 1.5 hours, and has high requirements for operators, so it is difficult to promote at the grassroots level

[0004] The prior art discloses a LAMP primer for detecting chicken. The primer can detect a limit of 50 fg of nucleic acid within a total reaction time of 60 minutes. The visualization method used is to observe whether the post-reaction system becomes turbid after the reaction is completed. Although this method is simple, due to individual differences and no standard interpretation range, it is easy to cause false positive or false negative results in the interpretation of results.

Another visualization method is to open the cover after the reaction and add dye to make it develop color. This process is extremely easy to cause product contamination, resulting in false positives for other detection indicators.

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