Myocardial damage marker MMPs (Matrix Metalloproteinases), TIMPs (Tissue Inhibitor of Metalloproteinases) and cTnI (Cardiac Troponin I) detection kit and detection method
A technology of myocardial injury and markers, applied in the field of medical detection, can solve the problems of not being able to meet the requirements of clinical rapid diagnosis, accurate diagnosis and large management needs, high price and high hardware requirements, so as to facilitate wide application and reduce detection The effect of high cost and sensitivity
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[0035] A kit for myocardial injury markers MMPs, TIMPs and cTnI, the kit mainly consists of fluorescent labeling probes, anti-MMPs, TIMPs and cTnI monoclonal antibodies, anti-mouse IgG antibodies, solid-phase membrane, and detection card;
[0036] Further, the fluorescent labeling probe is a nanoscale high-fidelity fluorescent labeling probe; its preparation method is: using the carboxyl group on the surface of the latex to connect to the amine group of the protein, and connecting the protein to the surface of the latex particle to prepare the fluorescent labeling probe ; When screening high-fidelity fluorescent signal latex, improve the stability of fluorescent signal by optimizing the ratio of reactants, connection method, temperature, time, medium, pH value, etc.;
[0037] Further, the anti-MMPs, TIMPs and cTnI monoclonal antibodies are fluorescent latex particle-labeled anti-MMPs, TIMPs and cTnI monoclonal antibodies, and the preparation method comprises:
[0038] 1) Disso...
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