Myocardial damage marker MMPs (Matrix Metalloproteinases), TIMPs (Tissue Inhibitor of Metalloproteinases) and cTnI (Cardiac Troponin I) detection kit and detection method

Abstract

The invention relates to the technical field of medical detection and in particular to a myocardial damage marker MMPs (Matrix Metalloproteinases), TIMPs (Tissue Inhibitor of Metalloproteinases) and cTnI (Cardiac Troponin I) detection kit and a detection method. The kit mainly consists of a fluorescence labeling probe, an anti-MMPs, TIMPs and cTnI monoclonal antibody, an anti-mouse IgG antibody, asolid-phase membrane and a detection card. According to the kit, MMps and TIMPs are firstly established and cTnI detection is combined for combined detection and diagnosis schemes of myocardial damage, the kit is prior to a conventional congestive heart failure marker group, accurate scientific bases are provided for clinical diagnosis of myocardial infarction and stroma reconstruction related congestive heart failure, secondly, by using an immunochromatography fluorescence quantitative determination detection method based on a fluorescence latex immunochromatography quantitative technique, MMPs, TIMPs and CtNI are combined and respectively detected, a myocardial damage detection system is established, immunochromatography is established, a fluorescence quantitative determination method is implemented, conventional euzymelinked immunosorbent assay (ELISA) is broken through, a myocardial damage detection system based on the fluorescence latex immunochromatography quantitative techniqueis established in China for a first time, and the kit has the characteristics of rapidness, accuracy, high sensitivity and good specificity.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a detection kit and detection method for myocardial injury markers MMPs, TIMPs and cTnI. Background technique [0002] In the past, it was believed that cardiac remodeling was only due to endogenous changes in cardiomyocytes, but now it is recognized that changes in the quantity, composition and structure of collagen in the extracellular matrix of cardiomyocytes are also involved in cardiac remodeling, that is, the process of matrix tissue repair and regeneration, and is the ventricular The important reason for remodeling, also known as myocardial matrix remodeling; myocardial matrix remodeling is one of the main factors that determine cardiac function and prognosis in patients with heart disease, and is the result of imbalance in the synthesis or degradation of cardiac matrix components. Plays an important role in maintaining the structural and functional integrity of ...

AI Technical Summary

Problems solved by technology

[0004] A number of studies have shown that MMPs and TIMPs are more sensitive and more accurate in reflecting the degree of myocardial injury in myocardial injury than previously detected markers; All the detection methods have many steps, slow detection speed, high hardware requirements, and the price is very expensive. The large-scale promotion and application of on-site first aid is also severely restricted; therefore, research on new detection methods that are simple and low-consumption and the development of new, low-cost, easy-to-use detection products have become important strategic issues for rapid diagnosis

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