Artificial propagation technical method of Oncorhynchus mykiss

A technology of artificial breeding and rainbow trout, which is applied in fish farming, climate change adaptation, and additional food elements, etc., can solve the problems of low seed production performance and survival rate, failure to establish an excellent breeding system, and lack of awareness of healthy breeding. Achieve the effects of promoting animal growth performance, regulating metabolism and immunity, high embryo survival rate and hatching rate

Active Publication Date: 2019-03-19
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The differences in production capacity and the market are due to reasons such as history, production methods and breeding techniques, but the most important ones are: lack of awareness of healthy breeding, failure to establish a good breeding sy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1:

[0032] Rainbow trout artificial reproduction technology methods, including injection of oxytocin, preparation of isotonic liquid, artificial egg collection, artificial insemination, eye hatching, fertilization rate detection, hatching of juveniles, seed intensive cultivation, and intensive cultivation of seedlings Enzymatically hydrolyzed porcine blood globulin powder is added to the bait; the preparation steps of the above-mentioned enzymatically hydrolyzed porcine blood globulin powder are:

[0033] a, provide anticoagulant pig blood;

[0034] b. Provide 4000U / g neutral protease based on the weight of anticoagulated pig blood;

[0035] c, based on the weight of anticoagulated pig blood, provide an accelerator containing sulfosalicylic acid and tea polyphenols;

[0036] d. After pre-treatment, the anticoagulant pig blood is contacted with the substances in b and c to form a hydrolyzed liquid, and the hydrolyzed liquid is spray-dried to obtain protein powder. Enzym...

Example Embodiment

[0050] Example 2:

[0051] The artificial propagation technique of rainbow trout includes the following steps:

[0052] 1) Select the sexually mature female fish to inject the oxytocin agent. After an interval of 52 hours, inject the oxytocin agent into the female and male fish at the same time. The first injection dose of the female fish is half of the second injection dose, and the amount of the male fish oxytocin agent is the female The second injection dose is half of the fish. The male fish are sexually mature and no longer inject oxytocin. The above oxytocins are PG, HCG and LRH for fish. The second injection dose of each oxytocin is PG7mg / kg and HCG1800 unit / kg, LRH18μg / kg;

[0053] 2) After being treated with a filter screen and a bio-cotton filter, sterilized by ultraviolet light and ozone as the matrix, dissolve sodium chloride, propylene glycol butyl ether and L-mandelic acid, and prepare the concentration to be sodium chloride Calculated as 0.95% isotonic solution, the...

Example Embodiment

[0063] Example 3:

[0064] The artificial propagation technique of rainbow trout includes the following steps:

[0065] 1) Select the sexually mature female fish to inject the oxytocin agent. After an interval of 50 hours, the female fish and the male fish are injected with the oxytocin agent at the same time. The first injection dose of the female fish is half the dose of the second injection, and the amount of the male fish oxytocin agent is the female The second injection dose of fish is half of the second injection. When the male fish is sexually mature, no oxytocin is injected. The above oxytocin is PG, HCG and LRH for fish. The second injection dose of each oxytocin is PG8mg / kg and HCG2000 unit / kg, LRH20μg / kg;

[0066] 2) After being treated with a filter and a bio-cotton filter, sterilized by ultraviolet light and ozone sterilized water as the matrix, dissolve sodium chloride, propylene glycol butyl ether and L-mandelic acid, and formulate the concentration to sodium chlorid...

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Abstract

The invention provides an artificial propagation technical method of Oncorhynchus mykiss and belongs to the technical field of fish propagation. The artificial propagation technical method of Oncorhynchus mykiss includes injecting an oxytocic, preparing an isotonic solution, performing artificial egg and semen collection, carrying out artificial semination, hatching eyed eggs, detecting fertilityrate, allowing larvae to emerge and hatch, and carrying out enhanced cultivation of fry, wherein enzymatically-hydrolyzed dried porcine blood cells are added to feed for the enhanced cultivation of fry; the enzymatically-hydrolyzed dried porcine blood cells are prepared via the steps of a, providing anti-coagulating porcine blood; b, providing neutral proteinase; c, providing an accelerant containing sulfosalicylic acid and tea polyphenols; d, pretreating the anti-coagulating porcine blood, allowing the blood to gain contact with the materials of steps b and c to form a hydrolysate, and spray-drying. The artificial propagation technical method of Oncorhynchus mykiss provided herein has the advantages that inter-egg adhesion can be relieved, egg fertility rate is increased, fertilized eggsare never contaminated by pathogens, the fertilize eggs are accelerated to enter a start phase, survival rate and hatching rate of fetuses are increased, and an initial domestication feed with good palatability and sensual experience is provided for assistance.

Description

technical field [0001] The invention belongs to the technical field of fish reproduction, and in particular relates to a technical method for artificial reproduction of rainbow trout. Background technique [0002] Rainbow trout belongs to the class of Osteichthyes, Herringiformes, Salmonidae, and Salmonidae. Rainbow trout has comprehensive and rich nutrition, delicious taste, no fishy smell, no small bones between muscles, and few spines. It has high edible value and is deeply loved by consumers. Rainbow trout is a cold-water fish of the Salmonidae family. It has the characteristics of long life, fast growth, and strong disease resistance. After years of domestication, it is easy to raise artificially. After the fish fry are gradually adapted, they can be cultivated and produced in most areas of northern my country. Through reasonable feeding and management, higher economic benefits can be obtained, so that rainbow trout has become one of the economic fishes strongly recomme...

Claims

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Application Information

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IPC IPC(8): A01K61/10A01K61/17A23K20/147A23K50/80
CPCA23K20/147A23K50/80A01K61/10A01K61/17Y02A40/81
Inventor 孙卫芳陈梦楠田云方张建设
Owner ZHEJIANG OCEAN UNIV
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