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Artificial propagation technical method of Oncorhynchus mykiss

A technology of artificial breeding and rainbow trout, which is applied in fish farming, climate change adaptation, and additional food elements, etc., can solve the problems of low seed production performance and survival rate, failure to establish an excellent breeding system, and lack of awareness of healthy breeding. Achieve the effects of promoting animal growth performance, regulating metabolism and immunity, high embryo survival rate and hatching rate

Active Publication Date: 2019-03-19
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The differences in production capacity and the market are due to reasons such as history, production methods and breeding techniques, but the most important ones are: lack of awareness of healthy breeding, failure to establish a good breeding system, only workshop-style management, no emphasis on broodstock breeding, no Strictly implement the operating procedures of artificial propagation technology, resulting in high production costs, low seed production performance and survival rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Rainbow trout artificial propagation technology methods, including injection of oxytocic agent, preparation of isotonic solution, artificial egg collection, artificial insemination, eye egg hatching, fertilization rate detection, juvenile hatching, seedling intensive cultivation, among which seedling intensive cultivation Enzymatically hydrolyzed porcine hemoglobin powder is added to the bait; the preparation steps of the above enzymolyzed porcine hemoglobin powder are as follows:

[0033] a, providing anticoagulated pig blood;

[0034] b, based on the weight of anticoagulated pig blood, provide 4000U / g of neutral protease;

[0035] c, based on the weight of anticoagulated pig blood, an accelerator comprising sulfosalicylic acid and tea polyphenols is provided;

[0036] d, the anticoagulated pig blood is subjected to pretreatment, and after being contacted with the substances in b and c, a hydrolyzate is formed, and the hydrolyzate is spray-dried to obtain protein powder...

Embodiment 2

[0051] The technical method for the artificial propagation of rainbow trout specifically comprises the following steps:

[0052] 1) Select sexually mature female fish to inject oxytocic agent, and inject oxytonic agent to female fish and male fish at the same time after an interval of 52 hours. Half of the dose for the second injection of the fish, male fish that are sexually mature will no longer be injected with oxytocic agents, the above-mentioned oxytocic agents are PG, HCG and LRH for fish, and the second injection doses of each oxytocic agent are PG7mg / kg, HCG1800 units / kg, LRH18μg / kg;

[0053] 2) Dissolve sodium chloride, propylene glycol butyl ether and L-mandelic acid with sterile water sterilized by ultraviolet light and ozone after being treated with a filter screen and a bio-cotton filter, and prepare a concentration equal to that of sodium chloride Calculated as 0.95% isotonic solution, the above-mentioned propylene glycol butyl ether and L-mandelic acid are res...

Embodiment 3

[0064] The technical method for the artificial propagation of rainbow trout specifically comprises the following steps:

[0065] 1) Select sexually mature female fish to inject oxytocic agent, and inject oxytonic agent to female fish and male fish at the same time after an interval of 50 hours. Half of the dose of the second injection of the fish, the male fish are sexually mature and no longer inject oxytocic agent, the above-mentioned oxytocic agent is PG, HCG and LRH for fish, the second injection dose of each oxytocic agent is PG8mg / kg, HCG2000 units / kg, LRH20μg / kg;

[0066] 2) Dissolve sodium chloride, propylene glycol butyl ether and L-mandelic acid with sterile water sterilized by ultraviolet light and ozone after being treated with a filter screen and a bio-cotton filter, and prepare a concentration equal to that of sodium chloride Calculated as 0.93% isotonic solution, the above-mentioned propylene glycol butyl ether and L-mandelic acid are respectively 0.8% and 0.7...

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Abstract

The invention provides an artificial propagation technical method of Oncorhynchus mykiss and belongs to the technical field of fish propagation. The artificial propagation technical method of Oncorhynchus mykiss includes injecting an oxytocic, preparing an isotonic solution, performing artificial egg and semen collection, carrying out artificial semination, hatching eyed eggs, detecting fertilityrate, allowing larvae to emerge and hatch, and carrying out enhanced cultivation of fry, wherein enzymatically-hydrolyzed dried porcine blood cells are added to feed for the enhanced cultivation of fry; the enzymatically-hydrolyzed dried porcine blood cells are prepared via the steps of a, providing anti-coagulating porcine blood; b, providing neutral proteinase; c, providing an accelerant containing sulfosalicylic acid and tea polyphenols; d, pretreating the anti-coagulating porcine blood, allowing the blood to gain contact with the materials of steps b and c to form a hydrolysate, and spray-drying. The artificial propagation technical method of Oncorhynchus mykiss provided herein has the advantages that inter-egg adhesion can be relieved, egg fertility rate is increased, fertilized eggsare never contaminated by pathogens, the fertilize eggs are accelerated to enter a start phase, survival rate and hatching rate of fetuses are increased, and an initial domestication feed with good palatability and sensual experience is provided for assistance.

Description

technical field [0001] The invention belongs to the technical field of fish reproduction, and in particular relates to a technical method for artificial reproduction of rainbow trout. Background technique [0002] Rainbow trout belongs to the class of Osteichthyes, Herringiformes, Salmonidae, and Salmonidae. Rainbow trout has comprehensive and rich nutrition, delicious taste, no fishy smell, no small bones between muscles, and few spines. It has high edible value and is deeply loved by consumers. Rainbow trout is a cold-water fish of the Salmonidae family. It has the characteristics of long life, fast growth, and strong disease resistance. After years of domestication, it is easy to raise artificially. After the fish fry are gradually adapted, they can be cultivated and produced in most areas of northern my country. Through reasonable feeding and management, higher economic benefits can be obtained, so that rainbow trout has become one of the economic fishes strongly recomme...

Claims

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Application Information

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IPC IPC(8): A01K61/10A01K61/17A23K20/147A23K50/80
CPCA23K20/147A23K50/80A01K61/10A01K61/17Y02A40/81
Inventor 孙卫芳陈梦楠田云方张建设
Owner ZHEJIANG OCEAN UNIV
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