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A method for evaluating the anti-stress ability of shrimp fry of Penaeus vannamei

An evaluation method and anti-stress technology, applied in application, climate change adaptation, fish farming, etc., can solve the problem of unknown disease resistance

Active Publication Date: 2021-06-15
HAINAN HAIYI AQUATIC PROD SEED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many factors that reflect the quality of seedlings, such as growth rate, uniformity, and stress resistance ability. The most prominent feature is the strength of stress resistance ability, which is directly related to disease resistance. , but farmers often rely on visual observation when selecting seedlings, which can only intuitively reflect the size and body color of seedlings, but have no way of knowing their actual disease resistance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for evaluating the anti-stress ability of Penaeus vannamei shrimp seedlings, said evaluation method comprising the following steps:

[0023] 1) Detect the water body from which the shrimp seedlings come from, and the detected indicators include salinity, water temperature and dissolved oxygen;

[0024] 2) Record the number of days for cultivating shrimp seedlings, the number of days for cultivating shrimp seedlings of PL5 is 14-15 days, and the number of days for cultivating shrimp seedlings of PL10 is 19-20 days;

[0025] 3) Adjust the water quality for the test, the water quality is transparent and sterile, the stress water quality of PL5 shrimp seedlings is salinity 15ppt, salinity difference >7ppt, total alkalinity is 150-180mg / L, water temperature is 29-30℃, dissolved oxygen >5mg / L; the stress water salinity of PL10 shrimp seedlings is 0ppt and 30ppt, the total alkalinity is 150-180mg / L, the water temperature is 29-30℃, and the dissolved oxygen is >5mg / L; ...

Embodiment 2

[0034] 16 million seedlings were raised in workshop A of a certain seedling farm, cultivated for 14 days, in 8 ponds (No. 1-8#), each pond has a water body of 13T, and 300-500 seedlings were taken from each pond for packing.

[0035] (1) determine that the seedling stage is P5 shrimp seedling according to its cultivation days number 14 days;

[0036] (2) Measure the temperature, salinity, and dissolved oxygen of the pool water. The temperature is 30°C, the salinity is 30ppt, the dissolved oxygen is 5mg / L, and the total alkalinity is 146mg / L. The water quality data shows that the water quality environment is relatively normal ;

[0037] (3) Prepare the test water, 1L / can, 8 cans in total, the water quality of the test water is adjusted, the salinity is 15ppt, the temperature is 29.5°C, the dissolved oxygen is 6mg / L, and the total alkalinity is 152mg / L;

[0038] (4) affix a corresponding label to each jar by the workshop pool number, pick 100 normal shrimp seedlings in each sam...

Embodiment 3

[0044] 8 million seedlings were raised in Workshop B of a seedling farm, and they were cultivated for 19 days. There were 4 ponds (3-6# ponds), each pond had a water body of 13T, and 300-500 seedlings were taken from each pond for packaging.

[0045] (1) Determine that the seedling stage is P10 shrimp seedling according to its cultivating days number 18 days;

[0046] (2) Measure the temperature, salinity, and dissolved oxygen of the pool water. The measured temperature is 30°C, the salinity is 24ppt, the dissolved oxygen is 4.5mg / L, and the total alkalinity is 140mg / L. The water quality data shows that the water quality environment is relatively normal;

[0047] (3) Prepare the first group of test water, 1L / can, 4 cans in total, water salinity 0ppt, temperature 29.5°C, dissolved oxygen 5.8mg / L, total alkalinity 153mg / L;

[0048] (4) affix corresponding labels to each jar by the number of the workshop pool, pick 100 normal shrimp seedlings for each sample and put them into th...

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Abstract

The invention relates to an evaluation method, in particular to an evaluation method for the anti-stress ability of Penaeus vannamei shrimp seedlings. The evaluation method includes detecting the water body from which the shrimp seedlings are derived, recording the number of days for culturing the shrimp seedlings, and adjusting the water quality used for the test. , test the anti-stress survival rate of PL5 shrimp seedlings, and calculate the anti-stress survival rate of PL5 shrimp seedlings; test the anti-stress survival rate of PL10 shrimp seedlings, and calculate the anti-stress survival rate of PL10 shrimp seedlings; The survival rate of seedlings reflects the difference in seed quality, objectively reflects the stress resistance of Penaeus vannamei seedlings, provides a strong basis for seed quality selection, and can also improve the adaptability to changing breeding conditions and later disease resistance. The ability to provide a strong data basis for farmers to select seedlings to provide a strong basis.

Description

technical field [0001] The invention relates to an evaluation method, in particular to an evaluation method for the anti-stress ability of Penaeus vannamei shrimp seedlings. Background technique [0002] Penaeus vannamei, whose scientific name is Litopenaeus vannamei, has become a hotspot for farming because of its advantages such as fast growth, strong disease resistance, suitable temperature, suitable salt, and wide range. At present, the number of domestic seedlings has exceeded 600 billion, and there are many varieties; among them, the first-generation seedlings imported from abroad account for the main advantage; domestic second-generation seedlings and multi-generation seedlings also have certain characteristics. [0003] With the increasingly harsh domestic breeding environment, the distinction between good and bad seed quality is becoming more and more obvious. Due to the different quality characteristics of various seedlings, how to choose seedlings has become the p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/59
CPCA01K61/59Y02A40/81
Inventor 戴小连黄明
Owner HAINAN HAIYI AQUATIC PROD SEED CO LTD