Combined preparation for constructing mouse cis-platinum inner ear injury model and constructed model

An injury model and mouse technology, applied in the medical field, can solve problems such as poor tolerance, and achieve the effect of avoiding death of mice and convenient operation.

Pending Publication Date: 2019-03-22
THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

However, due to the small size and light weight of the mice, the tolerance of the mice to high-dose cisplatin is very poor. Died due to severe nephrotoxicity and neurotoxicity after cisplatin injection

Method used

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  • Combined preparation for constructing mouse cis-platinum inner ear injury model and constructed model
  • Combined preparation for constructing mouse cis-platinum inner ear injury model and constructed model
  • Combined preparation for constructing mouse cis-platinum inner ear injury model and constructed model

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Embodiment 1

[0034] 1.1 Experimental animals

[0035] Twenty-six adult healthy CBA / CAJ A male rats (Jackson Lab, U.S.A.) with normal hearing, aged 8-12 weeks, and weighing 25-33 g were selected.

[0036] 1.2 Animal grouping and experimental protocol

[0037] 20 mice were divided into 2 groups according to random number method, 10 mice in each group;

[0038] (1) After general anesthesia with gas inhalation isoflurane (4% isoflurane induction, 1.0-1.5% isoflurane maintenance), mice in group A were given 200 mg / kg furosemide first (Phoenix Pharmaceutical INC. St Joseph, MO USA) Intraperitoneal injection, followed by intraperitoneal injection of 1.0 mg / kg of cisplatin (SigmaP-4394) after 1 h.

[0039] (2) After general anesthesia with gas inhalation isoflurane, mice in group B were given intraperitoneal injection of 200 mg / kg furosemide first, followed by intraperitoneal injection of 0.5 mg / kg cisplatin after 1 hour.

[0040]After intraperitoneal injection of cisplatin, all mice continued ...

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Abstract

The invention discloses a combined preparation for constructing a mouse cis-platinum inner ear injury model, the combined preparation comprises a cis-platinum injection and a furosemidum injection, the weight ratio of the cis-platinum in the cis-platinum injection to a laboratory mouse used for establishing the inner ear injury model is 0.4-1 mg/kg, and the weight ratio of the furosemidum in the furosemidum injection to the laboratory mouse used for establishing the inner ear injury model is 200-400 mg/kg. The invention further provides a method for constructing the mouse cis-platinum inner ear injury model by using the combined preparation. The combined preparation can establish the stable reliable mouse cis-platinum inner ear injury model, and the construction method is simple and is easy to operate.

Description

technical field [0001] The present invention relates to the field of medicine, more particularly, to a combined preparation for constructing a mouse cisplatin inner ear injury model and the constructed model. Background technique [0002] Cisplatin is one of the commonly used anti-tumor drugs in clinical practice, and has good anti-tumor effects on a variety of common tumors, such as bladder cancer, testicular cancer, cervical cancer, lung cancer, and head and neck cancer. However, in the course of clinical medication, a high proportion of patients experienced adverse reactions such as tinnitus, hearing loss and dizziness. The ototoxicity, nephrotoxicity and neurotoxicity of cisplatin have become one of the main reasons for limiting its clinical application. [0003] In order to understand the mechanism of inner ear injury caused by cisplatin, and to find effective drugs or therapeutic methods to prevent cisplatin ototoxicity, people use animal models to find the answer; an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/24A61K31/341A61K9/08A61D7/00
CPCA61D7/00A61K9/0019A61K9/08A61K31/341A61K33/24
Inventor 李永奇杨钦泰
Owner THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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