Deep sea micro-organism in situ enrichment sampling device

An in-situ enrichment and sampling device technology, which is applied in the method of sampling biological materials, the determination/inspection of microorganisms, the method of supporting/immobilizing microorganisms, etc. Achieve no pressure mutation, avoid sample contamination, and increase the number of results

Active Publication Date: 2019-04-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This solution is limited by the volume of the sampling container, and the number of microorganisms that can be collected at a time is very limited
In addition, the sampling container in the traditional scheme is filled with air before it is put into the predetermined depth, and the moment it is opened, the sample will have a huge pressure change, which may lead to the decomposition and loss of some components
Some improved sampler solutions avoid sudden pressure changes during sampling by pre-filling the container with distilled water and keeping the container open during lowering, but this in turn causes a small amount of seawater from other depths to enter the container and contaminate the sample

Method used

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  • Deep sea micro-organism in situ enrichment sampling device

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Embodiment Construction

[0022] The specific implementation manners of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0023] Such as figure 1 As shown, a sampling device for in-situ enrichment of deep-sea microorganisms includes a sampling cylinder 1; a deep-water pump 3, a deep-water motor 5 and a main frame 7;

[0024] The sampling cylinder 1 includes a cylindrical cylinder body 24 and cylinder covers at the front and rear sides, and two O-rings are used for sealing between the cylinder body 24 and the cylinder cover. The sampling cylinder 1 is horizontally installed on the sampling cylinder base 8 through the annular fixing ring 9 . The sampling cylinder base 8 is fixed on the front end of the main body frame 7 by bolts. The center of the front side cylinder cover 21 is provided with a through hole, which is connected to the front filter port 11 through the check valve 10, and the front filter port 11 is provided with a filter net for filt...

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Abstract

The present invention discloses a deep sea micro-organism in-situ enrichment sampling device. The deep sea micro-organism in-situ enrichment sampling device comprises a sampling cylinder, the samplingcylinder comprises a cylindrical cylinder body, a front side cylinder cover and a rear side cylinder cover, the sampling cylinder is horizontally arranged on a sampling cylinder base seat, a center of the front side cylinder cover is provided with a through-hole, the through-hole is connected with a front end filter port through a one-way valve, a bottom part of the rear side cylinder cover is provided with a through-hole, and the through-hole is connected with an inlet end of a deep water pump through a one-way valve; a bottom part of the deep water pump is fixedly arranged on a main body frame by bolts; the deep water pump is driven by a deep water motor; and the cylinder body is provided with a plurality of stacked filter modules, the filter module comprises an upper part filter membrane support frame and a lower part filter membrane support frame connected by bolts, and a biological filter membrane is arranged between a bottom surface of the upper part filter membrane support frame and a top surface of the lower part filter membrane support frame. The deep sea micro-organism in-situ enrichment sampling device can realize deep sea microorganism in-situ enrichment sampling, canalso maintain pollution-free and pressure mutation-free sampling processes, and has advantages of simple structure, convenient operation, etc.

Description

technical field [0001] The invention relates to a sampling device, in particular to a sampling device for in-situ enrichment of deep-sea microorganisms. Background technique [0002] The extreme environment of low temperature and high pressure in the deep sea has created its unique biological resources. Previous studies have shown that deep sea microorganisms have extremely high abundance, activity, composition and functional diversity, and have formed special species, gene types and Metabolites have important scientific research and economic value. Traditional deep-sea microbial sampling devices generally use simple open containers, which are placed in a predetermined depth for sampling and recovery. This solution is limited by the volume of the sampling container, and the number of microorganisms that can be collected at a time is very limited. In addition, the sampling container in the traditional solution is filled with air before it is put into the predetermined depth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C12M1/12
CPCC12Q1/24
Inventor 陈家旺何巍涛王玉红肖婧王豪黄越孙瑜霞
Owner ZHEJIANG UNIV
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