Sampling device for in-situ enrichment of deep-sea microorganisms

A technology of in-situ enrichment and sampling device, which is applied in the direction of biological material sampling method, microorganism measurement/inspection, method of supporting/immobilizing microorganisms, etc. It can solve the problems of sample pollution, component decomposition loss, and limited number of microorganisms, etc. Achieve the effect of avoiding sample contamination and increasing the quantity

Active Publication Date: 2022-03-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This solution is limited by the volume of the sampling container, and the number of microorganisms that can be collected at a time is very limited
In addition, the sampling container in the traditional scheme is filled with air before it is put into the predetermined depth, and the moment it is opened, the sample will have a huge pressure change, which may lead to the decomposition and loss of some components
Some improved sampler solutions avoid sudden pressure changes during sampling by pre-filling the container with distilled water and keeping the container open during lowering, but this in turn causes a small amount of seawater from other depths to enter the container and contaminate the sample

Method used

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  • Sampling device for in-situ enrichment of deep-sea microorganisms
  • Sampling device for in-situ enrichment of deep-sea microorganisms

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Embodiment Construction

[0022] The specific implementation manners of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0023] Such as figure 1 As shown, a sampling device for in-situ enrichment of deep-sea microorganisms includes a sampling cylinder 1; a deep-water pump 3, a deep-water motor 5 and a main frame 7;

[0024] The sampling cylinder 1 includes a cylindrical cylinder body 24 and cylinder covers at the front and rear sides, and two O-rings are used for sealing between the cylinder body 24 and the cylinder cover. The sampling cylinder 1 is horizontally installed on the sampling cylinder base 8 through the annular fixing ring 9 . The sampling cylinder base 8 is fixed on the front end of the main body frame 7 by bolts. The center of the front side cylinder cover 21 is provided with a through hole, which is connected to the front filter port 11 through the check valve 10, and the front filter port 11 is provided with a filter net for filt...

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Abstract

The invention discloses a sampling device for in-situ enrichment of deep-sea microorganisms, which comprises a sampling cylinder. The sampling cylinder includes a cylindrical cylinder body and cylinder covers on the front and rear sides. There is a through hole, which is connected to the front filter port through a one-way valve, and a through hole is provided at the bottom of the rear cover, which is connected to the inlet end of the deep-water pump through a one-way valve; the bottom of the deep-water pump is fixed on the main frame by bolts Above; the deep water pump is driven by a deep water motor; there are several stacked filter membrane modules in the cylinder, the filter membrane modules include the upper filter membrane support and the lower filter membrane support connected by bolts, the bottom surface of the upper filter membrane support and the lower filter membrane A biofiltration membrane is arranged between the top surfaces of the membrane supports. The invention can realize the in-situ enrichment sampling of deep-sea microorganisms, and can keep the sampling process free from pollution and pressure mutation, and has the advantages of simple structure, convenient operation and the like.

Description

technical field [0001] The invention relates to a sampling device, in particular to a sampling device for in-situ enrichment of deep-sea microorganisms. Background technique [0002] The extreme environment of low temperature and high pressure in the deep sea has created its unique biological resources. Previous studies have shown that deep sea microorganisms have extremely high abundance, activity, composition and functional diversity, and have formed special species, gene types and Metabolites have important scientific research and economic value. Traditional deep-sea microbial sampling devices generally use simple open containers, which are placed in a predetermined depth for sampling and recovery. This solution is limited by the volume of the sampling container, and the number of microorganisms that can be collected at a time is very limited. In addition, the sampling container in the traditional solution is filled with air before it is put into the predetermined depth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/26C12M1/12
CPCC12Q1/24
Inventor 陈家旺何巍涛王玉红肖婧王豪黄越孙瑜霞
Owner ZHEJIANG UNIV
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