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Light source modulation method of confocal scanning microscope

A light source modulation and microscope technology, which is applied in the field of confocal scanning optical imaging instruments and can solve problems such as insufficient image quality.

Pending Publication Date: 2019-04-02
BRIGHTVIEW MEDICAL TECH NANJING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The result of this problem is that in order to get a good enough sample image signal, the sides of the sample must receive 7 times more light dose than the middle of the sample
If only 1 / 7 of the dose is used to ensure that the images on both sides of the sample have a good enough signal-to-noise ratio, then the middle of the sample must be underexposed and the image quality cannot meet the specified requirements.

Method used

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  • Light source modulation method of confocal scanning microscope
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  • Light source modulation method of confocal scanning microscope

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] refer to Figure 2A In the image window 111 and the image window 121, the relatively linear part between the scanning window 11 and the scanning window 12 is taken. Since the sample image is severely stretched and distorted on both sides of the sample with a low speed of the fast resonant mirror, it will cause the image to be unsuitable.

[0065] In the above-mentioned embodiment 1, when a super luminescent diode (SLD, Super Luminescent Diode) is used as the imaging light source, this method is relatively easy to implement. A typical SLD usually has an opto-isolator with a built-in bandwidth of 100kHz to 200kHz, while the resonant frequency of a common fast resonant mirror is generally between 4kHz and 16kHz. Therefore, the SLD has a bandwidth of 100 kHz to 200 kHz, which is fast enough to reflect the switching frequency from the fast resonant mirror. According to the Nyquist sampling theory, it can be guaranteed that no sampling distortion will occur.

[0066] refer t...

Embodiment 2

[0073] The method adopted in this embodiment is to use a light source power modulator, and a typical application is to use an Acousto-Opto Modulator (AOM, Acousto-Opto Modulator) to modulate the light source. The modulation method starts from formula (5). In the formula (5), if the transmitting end power P of the light source 0 After AOM, and then perform nonlinear modulation on AOM, get:

[0074]

[0075] Among them, k is the attenuation coefficient of the AOM, which is a constant. Substitute formula (6) into formula (5) to get:

[0076] I'(x)=ΔI(x) / Δx=k·P 0 / ω (7)

[0077] That is to say, after the power modulation of the formula (6), the radiation distribution of the light source in the sample space becomes a constant.

[0078] Simplify formula (6) further to get,

[0079]

[0080] refer to Figure 4A , which shows that simultaneous power modulation 21, 22 is performed on the output end of the light source in the forward scanning sampling window 11 and the reve...

Embodiment 3

[0088] The method adopted in this embodiment is to combine the methods of Embodiment 1 and Embodiment 2 at the same time, and at the same time turn off the SLD light source outside the image window, and then superimpose a power modulator, such as an AOM, to perform power modulation on the output end of the light source.

[0089] Such as Figure 6A As shown, simultaneous power modulation 211, 221 and light source switch modulation 311, 321 are performed on the light source in the forward scanning image window 111 and reverse scanning image window 121 of the fast resonant mirror through the AOM.

[0090] Such as Figure 6BAs shown, simultaneous power modulation 211 and light source switching modulation 311 are performed on the light source in the forward scanning image window 111 of the fast resonant mirror through the AOM.

[0091] Such as Figure 6C As shown, simultaneous power modulation 221 and light source switching modulation 321 are performed on the light source in the ...

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Abstract

The invention discloses a light source modulation method of a confocal scanning microscope. The method comprises: taking an intermediate linear part of a scanning window at an image window; and according to a quick resonance scanning mirror window and an image acquisition window, generating a modulation signal by using electronic hardware to realize real-time turning on or off of an imaging lightsource. Therefore, the light source power in a fast scanning direction is modulated to enable the radiation quantity of the light source to meet a linear distribution requirement on the biological sample surface; and the light source is turned off at the scanning area without data sampling, so that the light radiation amount of the sample surface is minimized.

Description

technical field [0001] The present invention relates to confocal scanning optical imaging instrument technology, in particular to a confocal scanning microscope light source modulation method, which can be used for two-dimensional scanning of biological samples (such as skin, fundus and other biological tissues, which can be referred to as "sample" for short) , to obtain high-definition two-dimensional images of biological samples. Background technique [0002] The confocal scanning microscopy optical system usually uses a fast resonant scanning mirror and a slow linear scanning mirror in the orthogonal direction to perform two-dimensional scanning of the sample to obtain a two-dimensional image of the sample. [0003] Figure 1A shows how a typical scanning mirror works. Such as Figure 1A As shown, the fast resonant mirror 11 continuously scans the sample in the first direction in a sinusoidal manner, wherein any sinusoidal scanning cycle can be decomposed into forward sc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/00G02B21/06H05B33/08H05B44/00
CPCG02B21/0032H05B45/00Y02B20/30
Inventor 张杰张金莲杭荟
Owner BRIGHTVIEW MEDICAL TECH NANJING CO LTD
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