A kind of composite bacterial agent for black and odorous water body and its preparation method and application
A black and odorous water body and composite bacterial agent technology, which is applied in the direction of biochemical equipment and methods, chemical instruments and methods, and methods based on microorganisms, can solve problems such as unsatisfactory treatment effects, single microbial effects, and unstable timeliness. Achieve the effect of eliminating peculiar smell, improving water transparency and promoting rapid reproduction
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[0032]The preparation process of each bacterial strain bacterium liquid used in the embodiment of the present invention is as follows:
[0033] (1) Four strains of Pseudomonas slough, Pseudomonas alcaligenes, Paracoccus denitrificans and Micrococcus lyticus were picked and inoculated into solid slant medium for cultivation, the cultivation temperature was 28°C, and the cultivation time was After 24 hours, after the growth of colonies on the slant medium, inoculate it into the liquid medium for shaker culture, the shaker speed is 150-180r / min, the culture temperature is 28-30°C, and the culture time is 24-36h , when the number of live bacteria reaches 1×10 8 When the CFU / mL was reached, the preparation of the bacterial solution was completed, and the bacterial solutions of Pseudomonas saccharides, Pseudomonas alcaligenes, Paracoccus denitrificans and Micrococcus lysolyticus were obtained respectively.
[0034] The solid slant medium is prepared by mixing the following raw mate...
Embodiment 1
[0039] Mix the bacterial solutions of Pseudomonas sludge, Pseudomonas alcaligenes, Geobacillus stearothermophilus, Paracoccus denitrificans and Micrococcus lyticus at a volume ratio of 1:2:3:3:1, and then After high-temperature sterilization, inoculate with a volume ratio of 10% of the fermentation medium, ferment at 30°C for 5 days, and when the number of viable bacteria reaches 5×10 7 When the CFU / mL is above, through centrifugal filtration and freeze-drying at -21°C until the water content is less than or equal to 5%, the composite bacterial agent for treating black and odorous water can be obtained. The fermentation medium is made by mixing the following raw materials in weight ratio: brown sugar 15-25g, peptone 9-10g, beef extract 3-5g, NaCl 2-5g, KH 2 PO 4 0.75-1.5g, K 2 HPO 4 0.8-1g, supplemented with distilled water to 1L, pH = 7, sterilized at 121°C for 30min and cooled before use.
[0040] The composite bacterial agent is mainly used for the treatment of black a...
Embodiment 2
[0046] Others are the same as in Example 1, with the water body 2.5 / 10000 adding the composite bacterial agent of the present invention, taking water samples for testing every three days, and observing the changes in water quality. The experimental results show that after adding the composite bacterial agent of the present invention, the phenomenon of black odor is obviously weakened on the 6th day, the transparency of water quality is improved, and the peculiar smell of the water body is obviously weakened. After 18 days, COD, NH 3 The removal rates of -N and TP content reached 91.08%, 74.11% and 91.85%, respectively. For removal see figure 2 , the water quality indicators of each stage after treatment are shown in Table 2.
[0047] Table 2 Various indicators of water quality in different stages of the black and odorous river treatment
[0048] governance stage COD (mg / L) NH 3 -N (mg / L)
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