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A Shewanella and its Application in Controlling Vegetable Soil Nitrogen Pollution

A technology of Shewanella and soil nitrogen, applied in the field of soil nitrogen pollution control, to promote plant growth, improve farmland ecological environment, and reduce pollution problems

Active Publication Date: 2021-05-25
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the above-mentioned problem of soil nitrogen pollution and provides a Shewanella bacterium and its application in controlling vegetable soil nitrogen pollution. The Shewanella bacterium has the characteristic of promoting crop growth

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Isolation, Culture, Screening and Purification of Shewanella A0B14

[0031] The sample was the rhizosphere soil of the greenhouse of the Hongqiang Vegetable Production and Marketing Professional Cooperative in Lanling County, Shandong Province. The collected soil was put into a sealed bag and recorded.

[0032] After the soil samples were brought back to the laboratory, 0.1 g of soil samples were added to 5 mL of PBS buffer, fully shaken and mixed, centrifuged at 2000 g to take the supernatant, and diluted 10 times to 10 -8 , then take 10 -4 to 10 -7 The diluted solution was spread on LB plate, and after culturing in a 28°C incubator for 36 hours, a single colony was picked and streaked on a new LB plate, and the streaked single colony was picked and stored on an LBA plate.

[0033] Prepare reagents A and B, A is sulfanilic acid 5 mol / L (acetic acid solution 100 mL), B is α-naphthylamine 5 mol / L (acetic acid solution 100 mL), take a part of the pure strain and inocula...

Embodiment 2

[0038] Shewanella Shewanella Growth-promoting effect of sp. A0B14 on pepper

[0039] Use sterile soil to grow peppers in flowerpots, and conduct experiments when the height of the plants is about 7 cm.

[0040] The preparation method of Shewanella AOB14 fermentation broth, the concrete steps are as follows:

[0041] (1) Seed cultivation

[0042] Preparation of first-class seeds: the medium is LB medium, the ingredients are peptone 10g, sodium chloride 10g, yeast extract 5g, agar 10g, pH adjusted to 7.0-7.2, add water to make up to 1000 mL, sterilize at 121 °C 15min standby; liquid medium LB side composition is similar to solid medium, but without adding agar. After AOB14 was inoculated into 200ml of the above LB medium, cultured at 180rpm / min and 30°C overnight to OD 600 is 0.6-0.8, and the above-mentioned culture medium is used as the first-class seed.

[0043] Preparation of secondary seeds: use medium as LB medium, the ingredients are peptone 10g, sodium chloride 10g, ...

Embodiment 3

[0048] Shewanella Shewanella The control effect of sp. A0B14 soil nitrogen pollution control bacteria on vegetable soil nitrogen pollution

[0049] The preparation method of Shewanella AOB14 fermentation broth, the concrete steps are as follows:

[0050] (1) Seed cultivation

[0051] Preparation of first-class seeds: the medium is LB medium, the ingredients are peptone 10g, sodium chloride 10g, yeast extract 5g, agar 10g, pH adjusted to 7.0-7.2, add water to make up to 1000 mL, sterilize at 121 °C 15min standby; liquid medium LB side composition is similar to solid medium, but without adding agar. After AOB14 was inoculated into 200ml of the above LB medium, cultured at 180rpm / min and 30°C overnight to OD 600 is 0.6-0.8, and the above-mentioned culture medium is used as the first-class seed.

[0052] Preparation of secondary seeds: use medium as LB medium, the ingredients are peptone 10g, sodium chloride 10g, yeast extract 5g, agar 10g, pH adjusted to 7.0-7.2, add water to...

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Abstract

The invention relates to the technical field of soil nitrogen pollution control, in particular to a Shewanella bacterium and its application in controlling vegetable field soil nitrogen pollution. The Shewanella preservation number is CGMCC No.16843, and the preservation date is November 30, 2018. The present invention also discloses the molecular biological characteristics and cultivation methods of the above-mentioned strains, and verifies that it has the characteristics of promoting crop growth, and It is further used as a soil conditioner to control vegetable soil nitrogen pollution.

Description

technical field [0001] The invention relates to the technical field of soil nitrogen pollution control, in particular to a Shewanella and its application in controlling soil nitrogen pollution in vegetable fields. Background technique [0002] my country is a traditional agricultural country. In order to pursue high yield of vegetable crops, farmers often use a large amount of nitrogen fertilizer in vegetable fields. The growth cycle of vegetables is short, and the frequency of fertilization and irrigation is higher than that of other crops. For a long time, the amount of nitrogen input in the soil of vegetable fields is far greater than the demand of crops, which leads to the accumulation of nitrogen content in the soil, especially the nitrate in the soil of vegetable fields. The nitrogen content increased sharply. In addition, nitrogen accumulation leads to a decrease in the nitrogen absorption and utilization rate of crops, and at the same time, nitrate nitrogen is not e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00B09C1/10C12R1/01
CPCA01N63/00B09C1/10C12N1/20C12N1/205C12R2001/01Y02P60/21
Inventor 周方园张新建谢雪迎吴晓青赵晓燕周红姿张广志范素素
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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