A method for rapid extraction of rna using an animal and plant tissue ablation instrument based on the principle of ultrasound

Abstract

A method for rapidly extracting RNA using an ultrasonic principle-based animal and plant tissue ablation instrument, comprising: 1) first preserving tissues or cells using an extracting liquid A and then adding an extracting liquid B into the tissues or cells preserved using the extracting liquid A, and uniformly mixing to obtain a mixture; 2) performing ultrasonic crushing on the mixture when extracting RNA from the tissues; 3) directly performing the operation in step 5) below when extracting RNA from the cells; 4) uniformly mixing the mixture subjected to ultrasonic crushing by inversion; 5) in the mixture of RNA extracted from the cells in step 3) or the mixture of step 4), adding chloroform for centrifugation, and taking an upper layer of liquid; 6) adding isopropanol into the upper layer of liquid, and then performing centrifugation to obtain a precipitate; 7) adding an ethanol solution into the precipitate, uniformly mixing, centrifuging, and discarding the liquid; 8) finally, adding water for dissolution.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and in particular relates to a method for rapidly extracting RNA by using an animal and plant tissue ablation instrument based on the principle of ultrasound. Background technique [0002] In modern molecular biology experiments and clinical molecular diagnosis, it is often necessary to isolate and purify RNA from tissues and cells, and the quality of RNA often affects the success or failure of molecular biology experiments such as cDNA library, RT-PCR and Northern Blot. [0003] The commonly used method is to first protect the tissue with a tissue protection solution, and then use the extraction solution to extract the RNA in the tissue or cells. Commonly used protection methods include liquid nitrogen protection and protection solution protection, and the commonly used protection solutions include RNA later reagents and RNA safety reagents. [0004] Among them, the liquid nitro...

AI Technical Summary

Problems solved by technology

The liquid nitrogen grinding method requires a large tissue block, and it is difficult to completely break up the tissue block; the homogenization method is difficult to completely inhibit the activity of endogenous RNase, and it is easy to cause endogenous degradation; the disadvantage of the repeated freeze-thaw method is that it is difficult to Thoroughly lyses large tissues, and endogenous RNase activity is difficult to inhibit

Therefore, the RNA extraction of animal-derived tissue blocks has difficulties such as complicated operation, incomplete fragmentation, and difficulty in inhibiting the activity of endogenous RNA, which makes it difficult to guarantee the quality and yield of RNA extraction.

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