Nisin solid stabilizer development and application
A solid-solid, plate-and-frame filter press technology, applied in applications, peptides, organic chemistry, etc., can solve the problems of high production costs and achieve long-term stability, low production costs, and good repeatability
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Embodiment 1
[0024] 3 tons of Nisin fermentation broth, sterilized by ceramic membrane filtration, the obtained supernatant is concentrated 10 times with an ultrafiltration membrane greater than 1000 Daltons, 60kg of NaCl salted out is added in 300L of concentrated solution, and 80kg is obtained by plate-and-frame press filtration wet solids. Take 1g, 2g, 5g and 10g of Nisin plate and frame to filter the wet solids, respectively, to determine the content of Nisin.
[0025] Table 1 Nisin content (mg / g) in Nisin plate and frame press filtration wet solids
[0026]
[0027] The results in Table 1 show that the content of Nisin in the wet solids filtered by Nisin plate and frame is about 250 mg / g.
Embodiment 2
[0029] Accurately weigh 1.5kg of Nisin plate and frame press-filtered wet powder and 1.0kg of NaCl, add 0.1%, 0.5%, 1%, 2%, 3% of glycerol glucoside, dissolve in 10.0L aqueous solution, stir at room temperature for 15min, and then add concentration The pH was adjusted to 2.9 with hydrochloric acid, and the mixture was stirred evenly. Take 5L of Nisin solution for spray drying of Nisin products, the inlet temperature is 180°C, the outlet temperature is 80°C, and a blank control test is carried out at the same time.
[0030] In addition, 5L of Nisin solution was taken for vacuum drying of Nisin products. During the vacuum drying process, 40°C for 4h and 55°C for 4h to obtain a dry sample, which was ground and pulverized for content determination. The resulting product was subjected to a storage test at 55°C by spray drying and vacuum drying, and samples were analyzed at 7 days and 14 days, respectively. The content of Nisin was determined by the biological potency method, and t...
Embodiment 3
[0038] Accurately weigh 1.5kg of Nisin plate and frame press-filtered wet powder and 1.0kg of NaCl, add 0.1%, 1%, 2% of low-ester pectin, methionine and chitosan, dissolve in 10.0L aqueous solution, and stir at room temperature 15min, then add concentrated hydrochloric acid to adjust the pH to 2.9, after stirring evenly. Take 5L of Nisin solution for spray drying of Nisin products, the inlet temperature is 180°C, the outlet temperature is 80°C, and a blank control test is carried out at the same time.
[0039] In addition, 5L of Nisin solution was taken for vacuum drying of Nisin products. During the vacuum drying process, 40°C for 4h and 55°C for 4h to obtain a dry sample, which was ground and pulverized for content determination. The resulting product was subjected to a storage test at 55°C by spray drying and vacuum drying, and samples were analyzed at 7 days and 14 days, respectively. The content of Nisin was determined by the biological potency method, and the determinat...
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