Improved small culture device for filamentous fungi and culture method thereof

A technology for filamentous fungi and cultivation devices, which is applied in the field of improved small cultivation devices for filamentous fungi, which can solve the problems that it is difficult to observe complete fungal plane images, and achieve the effects of accelerating fungal spore production, convenient production, and clear images

Pending Publication Date: 2019-04-16
上海皓信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the defect that the existing small culture device is difficult to observe the complete fungal planar image under the microscope, and to provide an improved small culture device for filamentous fungi and its culture method

Method used

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  • Improved small culture device for filamentous fungi and culture method thereof
  • Improved small culture device for filamentous fungi and culture method thereof
  • Improved small culture device for filamentous fungi and culture method thereof

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Embodiment 1

[0052] This embodiment relates to an improved small culture device for filamentous fungi and its culture method; the device consists of a loading glass slide, a cover glass and a wet box.

[0053] Such as figure 1 , 3 As shown, a central groove 1 is provided in the middle of the loaded glass slide, and a peripheral groove 2 is arranged on the outer ring of the central groove 1. The peripheral groove 2 has a main body groove, is located at a corner of the main body groove and The first extension groove 3 connected with the edge of the loaded glass slide, and the second extension groove 4 located at the other side corner of the main body groove at a diagonal to the first extension groove 3, the first extension groove 3. The function of the second extension groove 4 is to provide sufficient gas passages for the fungal culture, and to prevent the formation of air bubbles when the dye solution is filled to prevent the dye solution from penetrating into the groove; in addition, due...

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Abstract

The invention discloses an improved small culture device for filamentous fungi and a culture method thereof. The device comprises a glass slide, a piece of cover glass and a wet box; a central grooveis formed in the middle of the glass slide, the central groove is externally and annularly provided with a peripheral groove, the peripheral groove is provided with a main body groove, a corner located at the main body groove, a first extending groove communicated with the edge of the glass slide and a second extending groove forming an opposite angle with the first extending groove. The device compresses filamentous fungi culture space in the narrow glass slide, the dye liquor flowing speed is limited, growing fungi can be shown in a plane, the morphological structures of the filamentous fungi after dyeing can be better protected, planer imaging is clear, and the effect of observing the morphology of the filamentous fungi under a microscope is better.

Description

technical field [0001] The invention relates to an improved small culture device for filamentous fungi and a culture method thereof. Background technique [0002] Small culture: That is, glass slide culture. Compared with the method of using petri dishes or test tubes for large fungal culture, small culture can effectively observe the phenomenon of fungal sporulation. [0003] Since Emile Rivalier and S. Seydel invented the small culture technology in 1932, the small culture technology has been continuously improved. At present, the steel ring method and the square method are commonly used in domestic small culture, but they are basically captured on the plane of the glass slide. . [0004] In the existing small culture device, there is a large space for fungi to grow, and it is difficult for fungi to adhere to the glass slide when they grow, and the flow of dye solution during staining can easily destroy the shape and structure of filamentous fungi, and it is difficult to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N1/14C12N3/00C12R1/645
CPCC12M23/02C12N1/14C12N3/00
Inventor 冯长海孙西钊王震臧蒙朱宇飞
Owner 上海皓信生物科技有限公司
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