A kind of fermentation method of rhodococcus and its application as adjuvant in animal vaccine
A technology of Rhodococcus erythrococcus and fermentation method, which is applied in the field of microbiology and immunology, and can solve problems such as induration, ulceration, and no obvious improvement of vaccine antibodies
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Embodiment 1
[0060] A method for fermenting Rhodococcus erythrococcus, specifically carried out according to the following steps:
[0061] 1) Preparation of eluted strains: Inoculate the Rhodococcus rubidococcus in a solid agar medium square bottle, and culture at 30°C for 4 days in aerobic or aerobic manner, until the cells in the square bottle appear light yellow or orange yellow or light red or deep red Afterwards, aseptically elute with an appropriate amount of normal saline, harvest the bacterial cells, and use them as eluted strains for later use;
[0062] 2) Preparation of the primary seed liquid: take the eluted strain prepared in step 1), inoculate the shake flask with 2% volume of the liquid culture medium, and cultivate it in a constant temperature shaker with continuous oscillation at a speed of 200 rpm, and cultivate it in aerobic or aerobic conditions at 30°C 48h, as a first-grade seed solution for subsequent use;
[0063] 3) Preparation of the secondary seed liquid: take th...
Embodiment 2
[0066] A method for fermenting Rhodococcus erythrococcus, specifically carried out according to the following steps:
[0067] 1) Preparation of eluted strains: Inoculate the Rhodococcus rubidococcus in a solid agar medium square bottle, and culture at 30°C for 4 days in aerobic or aerobic manner, until the cells in the square bottle appear light yellow or orange yellow or light red or dark red Finally, aseptically elute and harvest the thalline with an appropriate amount of normal saline, and use it as an eluting strain for subsequent use; the percentage by weight of the solid agar medium contained in the solid agar medium in the step 1) is: tryptone 1%, soybean peptone 0.5% , yeast extract powder 0.5%, NaCl 0.5%, sodium glutamate 0.5%, citric acid-citrate 0.25%, glycerin 2%, agar powder 1.5%;
[0068] 2) Preparation of the primary seed liquid: take the eluted strain prepared in step 1), inoculate the shake flask with 2% volume of the liquid culture medium, and cultivate it in...
Embodiment 3
[0072] A liquid culture medium specially used for fermenting and cultivating Rhodococcus Erythrococcus described in this application. The components and weight percentages of the liquid fermentation medium are: 1% tryptone, 1% soybean peptone, 0.1% urea, 1% yeast extract powder, NaCl 1%, sodium glutamate 1%, citric acid-citrate 0.5%, mannitol 5%, and the balance is water.
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