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A kind of biological material semi-solid storage medium and its application

A biomaterial and semi-solid technology, applied in the field of biomedical materials, can solve the problems of long-term preservation of biofilms at room temperature, and achieve the effects of low cost, convenient use and wide applicability

Active Publication Date: 2021-07-16
GUANGZHOU YUEQING REGENERATION MEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the existing problems currently used for long-term storage of biofilms at room temperature, the purpose of the present invention is to provide a semi-solid storage medium for biological materials

Method used

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  • A kind of biological material semi-solid storage medium and its application
  • A kind of biological material semi-solid storage medium and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Weigh 2.0g low-melting point agarose (gel temperature 30-35 ℃) and 0.1g dextran (molecular weight 40kDa) and disperse in 80ml PBS buffer solution with a concentration of 0.2mol / L, heat and stir continuously until completely swollen, add 0.1g glycerin, stir evenly, dilute the solution to 100ml with the same buffer solution, make the final pH 7.2~7.4, divide into storage containers, cool to below 38 ℃, put the biological cornea sample into the solution to expand and remove Bubbles, sealed and transferred to 4°C to refrigerate until the whole becomes a stable semi-solid state, sterilized by irradiation, and can be stored at room temperature for 1 to 2 years for a long time.

Embodiment 2

[0040] Weigh 0.2g low-melting point agarose (gel temperature 20-28 ℃) and 1g hydroxypropylmethylcellulose (viscosity 30000mPa s) and disperse in 40ml neutral HEPES buffer solution with a concentration of 0.01mol / L, heat Stir continuously until it swells completely, add 40g of polyethylene glycol 550, stir evenly, dilute the solution to 100ml with the same buffer solution, make the final pH 7.2~7.4, divide into storage containers, cool to below 28 ℃, put Spinal cord extracellular matrix samples were placed in the solution, unfolded and air bubbles were removed, sealed and transferred to 4°C for refrigeration until the whole became a stable semi-solid, sterilized by irradiation, and stored at 4°C for 1 year.

Embodiment 3

[0042] Weigh 1.2g of low-melting point agarose (gel temperature ~25°C) and 0.4g of hyaluronic acid (molecular weight: 80kDa) and disperse in 50ml of neutral citrate buffer solution with a concentration of 0.05mol / L, heat and stir continuously until completely swollen , add 20g of propylene glycol, stir evenly, dilute the solution to 100ml with the same buffer solution, make the final pH 7.2~7.4, divide it into a storage container, and put the decellularized pericardium sample into the solution when it is cooled to about 30°C Inside, unfold and remove air bubbles, seal and transfer to 4°C to refrigerate until the whole becomes a stable semi-solid, sterilized by irradiation, and can be stored at room temperature for 1 to 2 years.

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Abstract

The invention discloses a normal-temperature preservation solution, preparation method and application of a biofilm. The preservation solution includes low-melting point agarose, polysaccharide, structure protection agent and buffer solution. The preservation solution of the invention can wrap the preserved object when used Among them, the chain segment movement of the preserved substance is restricted, so that the biological extracellular matrix sample can be preserved at room temperature for a long time, or its storage temperature can be increased. The preparation method is simple, the use is convenient, the equipment and power consumption required for preservation can be reduced, the storage and transportation are convenient, the cost is reduced, and the economy of production and use is improved.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a semi-solid storage medium for biological materials and its application. Background technique [0002] Extracellular matrix medical materials have important clinical value, but the preservation of extracellular matrix is ​​still an insurmountable problem. Bone materials and other materials with high hardness and good structure retention can be kept for a long time under normal conditions after sterilization, and the shelf life can be further extended by freeze drying. However, soft bioactive materials such as corneal film, amniotic membrane, and heterogeneous mucous membrane must be stored in a wet state, otherwise the dehydration will cause the surface to shrink and collapse and seriously affect the effect. Even if the freeze-drying method that can better maintain the shape is used, It will also make its biologically active branch chains and sites shrink and int...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 易静楠别拓铭官习鹏
Owner GUANGZHOU YUEQING REGENERATION MEDICINE TECH CO LTD
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