Biochar-mediated solid biofilm anaerobic digestion promoter and preparation method thereof
An anaerobic digestion, biofilm technology, applied in biological sludge treatment, waste fuel, fixed on or in inorganic carriers, etc., can solve problems such as long start-up time, improve sufficiency, stabilize microorganisms, improve methane Yield effect
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Embodiment 1
[0042] A biochar-mediated solid biofilm anaerobic digestion accelerator, which is composed of 1 part of ferrous sulfate in solid form, 0.3 part of cobalt chloride, 1 part of nickel chloride, and 0.7 part of composite Vitamins, 97 parts of anaerobically digested solid biofilms; among them, the multivitamin consists of biotin, thiamine, vitamin B 12 composition.
[0043] A preparation method of a biochar-mediated solid biofilm anaerobic digestion accelerator, comprising the steps of:
[0044] S1 Preparation of anaerobic digestion solid biofilm:
[0045] S1.1 medium preparation
[0046] Medium: NH 4 Cl 1 g / L, MgCl 2 1 g / L, NaCl 0.5 g / L, K 2 HPO4 0.4 g / L, KH 2 PO4 0.4 g / L, peptone 2 g / L, yeast extract powder 2 g / L, sodium acetate 10 g / L, trace elements 10 mL, vitamin 10 mL, L-cysteine 0.5 g / L, 2‰ edge Azure 2 mL, distilled water 1000 mL (pH 7.0); among them,
[0047] Trace elements (g / L): N (CH2COOH) 3 4.5 (aminoacetic acid), FeCl 2 4H 2 O 0.4, MnCl H 2 O0.1, CoCl...
Embodiment 2
[0064] A biochar-mediated solid biofilm anaerobic digestion accelerator, which is composed of 1.5 parts of ferrous sulfate in solid form, 0.7 parts of cobalt chloride, 1.5 parts of nickel chloride, and 1.3 parts of composite Vitamins, 95 servings of anaerobically digested solid biofilms; the multivitamin consists of biotin, folic acid, riboflavin, vitamin B 12 composition.
[0065] A preparation method of a biochar-mediated solid biofilm anaerobic digestion accelerator, comprising the steps of:
[0066] S1 Preparation of anaerobic digestion solid biofilm:
[0067] S1.1 medium preparation
[0068] Medium: NH 4 Cl 1 g / L, MgCl 2 1 g / L, NaCl 0.5 g / L, K 2 HPO4 0.4 g / L, KH 2PO4 0.4 g / L, peptone 2 g / L, yeast extract powder 2 g / L, sodium acetate 10 g / L, trace elements 10 mL, vitamin 10 mL, L-cysteine 0.5 g / L, 2‰ edge Azure 2 mL, distilled water 1000 mL (pH 7.0); among them,
[0069] Trace elements (g / L): N (CH2COOH) 3 4.5 (aminoacetic acid), FeCl 2 4H 2 O 0.4, MnCl H ...
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