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Method for producing scleroglucan fermented liquid with Athena rosenbergii as bacterial species

A technology of Athena rosenbergii and scleroglucan, which is applied in the field of producing scleroglucan fermentation liquid, and can solve the problem of high impurity content in scleroglucan products, high impurities in scleroglucan fermentation liquid, and impurities in waste glucose mother liquor Many problems, to achieve the effect that is beneficial to the growth of bacteria, shorten the fermentation cycle, and facilitate the synthesis

Active Publication Date: 2022-05-31
HEBEI XINHE BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The patent literature with the application number 201310283916.8 discloses a method for producing scleroglucan fermentation broth using Athena rosenbergii and its main technical route is to inoculate Athena rosenbergii CGMCC No.7760 on waste molasses 1. The waste glucose mother liquor is used as a carbon source to carry out aerated fermentation to prepare scleroglucan, but waste molasses and waste glucose mother liquor are by-product wastes, and the effective sugar content is unstable, resulting in complex processes and difficult control of concentration during fermentation.
And waste molasses, waste glucose mother liquor are greatly affected by the region, and the supply is insufficient. In addition, the waste molasses and waste glucose mother liquor treatment process in the above-mentioned prior art is complicated and the quality is not easy to guarantee. The fermentation broth contains a lot of impurities, which increases the burden of the post-extraction process, resulting in high impurity content in scleroglucan products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] The present embodiment includes the following process steps:

[0068] The method for producing scleroglucan fermentation broth with Athelia roche as the strain is to inoculate the Athelia rolfsii CGMCC No.7760 (Athelia rolfsii) as the strain into a sterile sterile material containing carbon sources, necessary nitrogen sources and nutrients. In the nutrient solution, the pH of the nutrient solution is adjusted, and aeration and stirring fermentation is performed to obtain a scleroglucan fermentation broth; the aseptic nutrient solution is composed of the following raw materials:

[0069] Corn flakes sugar 80g / L; pea protein 3g / L; KH 2 PO 4 1.0g / L; MgSO 4 ·7H 2 O 0.3g / L; citric acid 1.3g / L; the balance is sterile water; pH=5.0-6.0.

[0070] Wherein the technological condition of each technological step in the production method is as follows:

[0071] The conditions for aeration and stirring fermentation are:

[0072] 0 hours-10 hours: ventilation volume 0.2vvm;

...

Embodiment 2

[0099] The difference between this embodiment and Embodiment 1 is:

[0100] In the present embodiment, the sterile culture liquid is made up of the following raw materials:

[0101] Corn flakes sugar 100g / L; pea protein 5g / L; KH 2 PO 4 2.5g / L; MgSO 4 ·7H 2 O 0.4g / L; citric acid 1.5g / L; the balance is sterile water; pH=5.0-6.0.

[0102] Wherein the technological condition of each technological step in the production method is as follows:

[0103] The conditions for aeration and stirring fermentation are:

[0104] 0 hours-10 hours: ventilation volume 0.3vvm;

[0105] 10 hours-20 hours: ventilation volume 0.5vvm;

[0106] 20 hours-30 hours: ventilation volume 1.0vvm;

[0107] 30 hours-40 hours: ventilation volume 0.7vvm;

[0108] 40 hours - end of fermentation: ventilation volume 0.3vvm;

[0109] During the fermentation, the temperature was 29° C., the pressure was 0.05 MPa, and the pH was 5.0-6.0; the stirring speed was 150 rpm, and the stirring was stopped for 2 hours ...

Embodiment 3

[0126] The difference between this embodiment and Embodiment 1 is:

[0127] In the present embodiment, the sterile culture liquid is made up of the following raw materials:

[0128] Corn flakes sugar 90g / L; pea protein 4g / L; KH 2 PO 4 2.0g / L; MgSO 4 ·7H 2 O 0.35g / L; citric acid 1.4g / L; the balance is sterile water; pH=5.0-6.0.

[0129] Wherein the technological condition of each technological step in the production method is as follows:

[0130] The conditions for aeration and stirring fermentation are:

[0131] 0 hours-10 hours: ventilation volume 0.25vvm;

[0132] 10 hours-20 hours: ventilation volume 0.45vvm;

[0133] 20 hours-30 hours: ventilation volume 0.9vvm;

[0134] 30 hours-40 hours: ventilation volume 0.65vvm;

[0135] 40 hours - end of fermentation: ventilation volume 0.25vvm;

[0136] During the fermentation, the temperature was 28° C., the pressure was 0.04 MPa, and the pH was 5.0-6.0; the stirring speed was 130 rpm, and the stirring was stopped for 2 ...

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Abstract

The invention relates to microbial fermentation, in particular to a method for producing scleroglucan fermentation liquid by using A. rosenbergii as strain. The method includes inoculating A. rosenbergii CGMCCNo.7760 into a culture solution containing carbon sources, adding necessary nitrogen sources and nutrients, adjusting the pH of the culture solution, and performing aeration stirring fermentation and other process steps, wherein the culture solution uses corn floss sugar and pea protein as the main ingredient. The invention solves the problems of low yield, long fermentation period, great difficulty in post-extraction and the like existing in the prior art, and has the advantages of short fermentation period and high yield, and the prepared scleroglucan is better than the prior art in the temperature resistance performance index. products, low production costs and other advantages.

Description

technical field [0001] The invention relates to microbial fermentation, in particular to a method for producing scleroglucan fermentation liquid by using A. rosenbergii as strain. Background technique [0002] Sclerodextran, also known as Sclerotinia polysaccharide, is a high molecular weight, non-ionic branched glucan. It is a glucan with β-D-(1-3) glucopyranosyl as the main chain and β-D-(1-6) glucopyranosyl as the side chain for every three glucose. Sclerodextran can be obtained by pure culture fermentation of filamentous fungi of the genus Sclerotinia. It has excellent thickening, pseudoplastic, high temperature resistance, high salt and pH adaptability in a wide range, and can be widely used in petroleum, paint, ceramics, food and other industries. [0003] At present, microorganisms have produced scleroglucan. However, because scleroglucan is usually produced by fungal fermentation, the fermentation yield is not high, which leads to its high price, which seriously li...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/08C12N1/14C12R1/645
Inventor 张禹张国沛张少华贾敬敏
Owner HEBEI XINHE BIOCHEM
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