Fluorescence imaging system of a gene sequencer

Abstract

The invention discloses a fluorescence imaging system of a gene sequencer, comprising: an optical system; an image sensor, which includes a fluorescence detection unit group and an optical filter, the fluorescence detection unit group is installed on the optical path of the fluorescence transmitted by the optical system, and the fluorescence detection The unit group is formed by an array of several fluorescence detection units, and each fluorescence detection unit corresponds to each gene molecule on the gene sequencing chip; each fluorescence detection unit includes four adjacent pixels, and each fluorescence detection unit The unit is covered with an optical filter, and the optical filter includes four areas corresponding to the four picture elements one by one, and each area is used to transmit the fluorescence generated by the excitation of a fluorescent marker. The four bases carry a fluorescent marker respectively, and the fluorescence generated by the excitation of a fluorescent marker only passes through one area of ​​the filter, and the transmitted fluorescence is identified by the pixel to determine the type of the excited fluorescent marker. Then the type of the read base is judged. It has simple structure and high sequencing efficiency.

Description

A Fluorescence Imaging System for Gene Sequencer technical field [0001] The present invention relates to the technical field of gene sequencing, in particular to a fluorescence imaging system of a gene sequencer. Background technique Gene sequencing refers to the analysis of the base sequence of a specific DNA fragment, that is, adenine (A), thymine (T), cytoplasmic The arrangement of pyrimidine (C) and guanine (G). At present, the method of fluorescent labeling is commonly used for gene sequencing, gene sequencing The function of the imaging system in the instrument is to excite the fluorescent markers on the gene sequencing chip with a laser to generate fluorescence, and collect the fluorescence signals. No., four bases combine with different fluorescent labels to generate four different fluorescence bands to identify bases. A gene molecule contains multiple bases, and during sequencing, one of the bases will be connected with a fluorescent marker, and the fluores...

AI Technical Summary

Problems solved by technology

[0005] Existing time-division multiplexing and space-division multiplexing technologies all set optical filters in the optical path of the optical system to achieve light splitting, which requires multiple tube mirror branches with a complex structure, but only one tube mirror branch, passing through the camera pixel There is no report on the method of setting the pixel filter to realize spectral splitting for gene sequencing

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