Ceftiofur time-resolved fluorescence lateral flow immunoassay quantitative detection test strip

A time-resolved fluorescence, ceftiofur technology, applied in analytical materials, measuring devices, instruments, etc., can solve the problems of low sensitivity, large inter-batch variation, small stock displacement, etc., and achieve the effect of high sensitivity

Inactive Publication Date: 2019-05-28
DANYANG YITAI BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rapid detection products developed based on colloidal gold labeling technology have problems such as qualitative or semi-quantitative, and large batch-to-batch differences; although the batch-to-batch difference of colored latex microspheres has improved, the sensitivity is still low and can only be qualitative or semi-quantitative ; The sensitivity of immunochromatography based on fluorescein labeling technology has been greatly improved, and quantitative detection can also be performed, but because the sample contains a high fluorescent background signal and the stock shift is small, it will have a greater impact on the detection

Method used

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  • Ceftiofur time-resolved fluorescence lateral flow immunoassay quantitative detection test strip
  • Ceftiofur time-resolved fluorescence lateral flow immunoassay quantitative detection test strip
  • Ceftiofur time-resolved fluorescence lateral flow immunoassay quantitative detection test strip

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Embodiment Construction

[0035] 1. Preparation of Components of Test Strips

[0036] 1.1 Preparation of quality control microspheres Preparation of quality control microspheres coated with biotinylated γ-globulin (BGG)

[0037] ⑴ Preparation of biotinylated BGG

[0038] with 0.1 M NaCNBH 3 BGG (purchased from Pel-Freez Biological) was prepared into a 10 mg / mL solution, and Biotin-X-X-NHS (N-hydroxysuccinimide modified biotin, manufacturer: SIGMA, Product number: B3295) solution to 16.2 mg / mL, add Biotin-X-X-NHS solution to BGG solution according to the amount of 1 mg BBG protein and 5.4 μl Biotin-X-X-NHS solution, mix well and place overnight at 4°C. Dialysis was used to remove free unreacted biotin, and the dialysate was biotin-labeled protein dialysate buffer (0.1 M Tris, 0.3 M Nacl, 0.005 MEDTA-Na-2H 2 0, pH8.0). After dialysis, the protein concentration was determined by BCA method.

[0039] ⑵ Use the above-mentioned labeled protein to coat the aldehyde-modified fluorescent microspheres

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Abstract

The invention relates to a preparing method and application of a ceftiofur quick time-resolved fluorescence lateral flow immunoassay quantitative detection test strip. The test strip is composed of three parts of the Fusion5 membrane, the nitrocellulose membrane and the water absorbing paper. Through a competition law principle, time-resolved fluorescence microspheres are utilized as immune markers, and to-be-tested antigens are accurately and quickly detected quantitatively.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a detection method for harmful residues in food, in particular to a time-resolved fluorescent immunochromatographic test strip for quantitative detection of ceftiofur, and a preparation method and application thereof. Background technique [0002] Ceftiofur (ceftiofur), also known as Saidefu, is the first third-generation cephalosporin antibiotic exclusively for animals. In 1988, the FDA approved ceftiofur sodium for the treatment of bovine respiratory bacterial diseases, when the drug was first marketed in the United States. Since then, FDA has successively approved ceftiofur sodium, ceftiofur hydrochloride and ceftiofur crystalline free acid for the treatment of respiratory diseases in 1-day-old chickens, turkeys, pigs, beef cattle, dairy cows, horses, goats and sheep. Canada, Japan and some European countries have also officially approved it for the treatment ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/533G01N33/543
Inventor杜霞张淑雅洪霞
OwnerDANYANG YITAI BIOTECH DEV