A kind of spider silk protein bone nail and preparation method thereof

A spider silk protein and spider silk technology, applied in the field of biomedicine, can solve the problems of easy fatigue, low breaking strength, no osteoconductivity, etc., and achieve the effects of good human body affinity, good compatibility, and no immune rejection.

Active Publication Date: 2022-06-07
江西丝科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the degradation of materials such as polylactic acid (PLA) will form some acidic products, which will have a negative impact on human tissues.
These materials cause acidic buildup as they degrade and are not osteoconductive, thus failing to promote bone cell growth
This type of material degrades quickly and is not suitable for the repair of main load-bearing bones
In addition, the mechanical properties of such materials cannot fully meet the clinical needs of bone repair, and it is necessary to improve their mechanical properties by adding reinforcing fibers.
[0004] At the same time, PLA toughened ceramic-based materials are also used to prepare bone implants, which can achieve the same mechanical properties as the bone nail and increase the biocompatibility, but the bone nail cannot be completely degraded.
[0005] In addition, although bone screws made of calcium phosphate bone materials have degradability, good osteoconductivity, and biocompatibility, and calcium phosphate bone screws can bear compressive loads, they have low fracture strength, brittleness, and fatigue

Method used

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  • A kind of spider silk protein bone nail and preparation method thereof
  • A kind of spider silk protein bone nail and preparation method thereof
  • A kind of spider silk protein bone nail and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 of the present invention provides a method for preparing a spider silk protein bone nail, such as figure 1 As shown, the preparation method of the spider silk protein bone nail includes the following steps.

[0039] Step 1. Prepare spider silk protein solution.

[0040] The spider silk protein solution can be a solution of natural spider silk protein or a solution of genetically recombinant spider silk protein. Next, the recombinant spider silk protein is introduced as an example. First, the engineered bacteria that have undergone high-density fermentation and express recombinant spider silk proteins are dissolved in the first Tris-HCl (PH=8) / NaCl / Imidazole solution (Tris-HCl concentration 20 mM, NaCl concentration 150 mM, Imidazole range 5 mM), Bacteria are broken with a bacteria breaker. The broken bacteria were centrifuged, and the supernatant was collected; then the supernatant was incubated under a constant temperature water bath at 80°C for 24 hour...

Embodiment 2

[0056] In step 1, the first Tris-HCl (PH=8) / NaCl / Imidazole solution is a solution of Tris-HCl concentration 5mM, NaCl concentration 200mM, Imidazole range 10mM); the second Tris-HCl (PH=8) / NaCl / Imidazole solution is Tris-HCl concentration 5mM, NaCl concentration 200mM, Imidazole range 40mM solution; The third Tris-HCl (PH=8) / NaCl / Imidazole solution is Tris-HCl concentration 50mM, NaCl concentration 200mM, Imidazole range 300mM solution . Other operations are the same as in Example 1.

[0057] The spider silk protein bone nail prepared by the preparation method of the spider silk protein bone nail provided in Example 2 of the present invention has good affinity with the human body, has no immune rejection reaction, has good compatibility in human tissue, and has no toxic effect on the human body; The preparation method of the spider silk protein bone nail provided in the embodiment of the invention can load the inside and surface of the bone nail by adding functional drugs in...

Embodiment 3

[0062] In step 1, the natural spider silk protein was dissolved in water, frozen at -40°C, and vacuum-frozen in a vacuum freeze dryer for 250 hours, the vacuum pressure was 0.001mBar, and the temperature was -80°C; anhydrous natural spider silk was obtained protein, and then 1 part by weight of anhydrous natural spider silk protein is dissolved in 0.5 part by weight of hexafluoroisopropanol, and the dissolving temperature is 27.5 degrees Celsius to obtain the spider silk protein solution. Other steps are the same as in Example 1.

[0063]The spider silk protein bone nail prepared by the preparation method of the spider silk protein bone nail provided in Example 3 of the present invention has good affinity with the human body, has no immune rejection reaction, has good compatibility in human tissue, and has no toxic effect on the human body; The preparation method of the spider silk protein bone nail provided by the embodiment of the invention can load the inside and surface of...

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Abstract

The invention relates to the technical field of biomedicine. The invention provides a method for preparing a spider silk protein bone nail. The preparation method of the spider silk protein bone nail comprises the following steps: (1) preparing a spider silk protein solution; (2) injecting the spider silk protein solution into a mold, methanol Or ethanol treatment and drying to obtain spidroin protein bone nails. The preparation method of the spider silk protein bone nail provided by the present invention is simple to operate and can be produced industrially. The spider silk protein bone nail prepared by it has the advantages of high strength, high toughness, lightness, ultraviolet resistance, and biodegradability. It is an amino acid and a polypeptide, which is non-toxic to the human body.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a spider silk protein bone nail and a preparation method thereof. Background technique [0002] Bone nails are commonly used bone fixation devices during bone repair. Traditional bone nails are made of metal materials such as stainless steel, titanium and their alloys. Although these materials have the advantages of high strength and good toughness, after the patient recovers, a second operation is required to take out the metal bone nails and bring them to the patient. It is painful for a second time, and the cavity left after the second operation also leaves the patient with a potential fracture risk. [0003] At present, degradable materials such as polylactic acid (PLA) and polyglycolide-lactide (PLGA) have been used in clinical bone repair. Although bone nails made of absorbable polymer materials can overcome many defects of traditional bone nail materials. However, t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/04A61L31/14A61L31/16C12P21/00C07K1/16C07K1/14
Inventor 陶虎柳克银
Owner 江西丝科生物科技有限公司
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