Active compound in gastrodia elata and its preparation method and application

A compound, Gastrodia elata technology, applied in the field of medicine, can solve the problem of not forming a unified aging theory, and achieve the effect of delaying aging

Active Publication Date: 2020-11-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since the application of experimental methods to study aging at the end of the 19th century, many theories have been put forward, including the theory of programmed aging, the theory of somatic cell mutation, the theory of error disaster, the theory of free radicals, the theory of neuroendocrine, the theory of immune aging, etc., but they have not yet formed a unified theory. theory of aging

Method used

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  • Active compound in gastrodia elata and its preparation method and application
  • Active compound in gastrodia elata and its preparation method and application
  • Active compound in gastrodia elata and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1, the preparation of active compound 1 in Gastrodia elata, concrete steps are as follows:

[0045] (1) Put 1.2kg of dried rhizome of Gastrodia elata in 80% methanol aqueous solution, extract twice at room temperature, each time for 24 hours (shaking); after concentrating by suction filtration, 121 g of extract is obtained.

[0046] (2) Dissolving the obtained extract in water, and extracting the water-soluble matter with ethyl acetate to obtain two components of the water layer and the ethyl acetate layer.

[0047] (3) Separate the water layer by reverse silica gel column chromatography, using methanol: water solvent system as eluent, methanol: water solvent system by volume ratio 15:85, 20:80, 25:75, 30:70 , 35:65, 40:60, 70:30 and 100:0 were eluted in sequence, and 1.8 g of fractions eluted at a volume ratio of 35:65 and 30:70 were taken.

[0048] (4) The fraction obtained in step (3) is separated by forward silica gel column chromatography, dichloromethane: methano...

Embodiment 2

[0058] Active compound 1 prolongs replicative lifespan analysis in embodiment 2 Gastrodia elata

[0059] At present, the biological models used for anti-aging research mainly include yeast, fruit flies, nematodes and mice. In this example, Saccharomyces cerevisiae was selected as the active system for anti-aging research; because yeast is a single-celled eukaryote with a short life cycle, its complete genome data has been obtained, and it is currently a commonly used aging model organism.

[0060] At the same time, resveratrol is used as a positive control, which is a well-known small molecular compound that shows anti-aging effects in various animal models.

[0061] The steps of the analysis method are as follows:

[0062] (1) Take out the K6001 yeast strain from the refrigerator at -30°C, wash it three times with PBS, 5ml each time, and remove the glycerol therein; then add 1ml PBS, pipette to suspend it, and then add it to 5ml liquid medium (1% yeast powder , 2% peptone, ...

Embodiment 3

[0069] Active compound 1 prolongs the sequential lifespan analysis in embodiment 3 Gastrodia elata

[0070] (1) Day -3: Take out the YOM36 yeast strain from the -30°C refrigerator, wash with PBS three times, 5ml each time, and remove the glycerol; then add 1ml of PBS, pipette, dilute to a certain number of times and count with a cell counting plate.

[0071] (2) After the glass petri dish is sterilized, pour 20 mL of sterilized and high-temperature solid medium (1% yeast powder, 2% peptone, 2% glucose, 2% Agar powder), after the culture medium is solidified, add a certain volume of yeast solution according to the dilution factor, so that the number of yeast in each dish is about 200, spread it evenly with a spreader, and put it in a 28°C constant temperature incubator Cultured in medium for 2 days to restore growth ability.

[0072] (3) Day -1: Pick a single yeast colony from the solid medium and inoculate it into liquid medium (0.17% yeast nitrogen source (without amino acid...

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Abstract

The invention provides an active compound in gastrodiaelata and a preparation method and application thereof. The preparation method comprises the following steps of: pulverizing dried gastrodiaelatarhizome, placing in methanol aqueous solution, leaching, filtering, concentrating, separating and purifying an extract to obtain a phenol glycoside active compound. The compound can obviously prolongthe replicative life and the chronological life of yeast cells and has a potential anti-aging function. A research finds that the aim of anti-aging is fulfilled by regulating the expression of Sod1, Sod2, Skn7, Sir2 and Uth1 genes, and improving the antioxidant ability of yeasts, including improving the survival rate of yeasts under oxidative stress, reducing levels of active oxygen and malondialdehyde in yeast cells, and simultaneously increasing the activity of catalase and total glutathione peroxidase. The active compound can be used for preparing anti-aging drugs or health care products. The structural formula of the compound is shown in the description.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to an active compound in Gastrodia elata and a preparation method and application thereof. Background technique [0002] With the rapid increase of the aging population, the incidence of age-related diseases is also increasing, so finding anti-aging drugs has become a top priority. Since the application of experimental methods to study aging at the end of the 19th century, many theories have been put forward, including the theory of programmed aging, the theory of somatic cell mutation, the theory of error disaster, the theory of free radicals, the theory of neuroendocrine, the theory of immune aging, etc., but they have not yet formed a unified theory. theory of aging. It can be seen that aging is a very complicated process, and our country is rich in traditional Chinese medicine resources and has thousands of years of experience in medicine. Therefore, it is our advantage to find ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/203C07H1/08A61P39/00
Inventor 戚建华潘彦君刘倩林燕飞向兰
Owner ZHEJIANG UNIV
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