Unlock instant, AI-driven research and patent intelligence for your innovation.

Novel spirulina culture medium

A culture medium and spirulina technology, applied in the field of algae cultivation, can solve the problems of imperfect cultivation technology, restricting the development of spirulina industry, unstable quality, etc.

Pending Publication Date: 2019-06-14
ZHEJIANG OCEAN UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the early and middle stages of the development of the spirulina industry in my country, due to the imperfect breeding technology, the low yield and unstable quality of spirulina biological production have become the key factors restricting the development of the spirulina industry.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel spirulina culture medium
  • Novel spirulina culture medium
  • Novel spirulina culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] This implementation case shows a new type of spirulina culture medium according to the following steps:

[0020] Spirulina was cultured in seawater under different concentrations of the following major nutritional factors, see Table 1 for details:

[0021]

[0022] Selected metal ion Ca 2+ , Mg 2+ , Fe 3+ and NaCl as the main variable factor, HCO 3 - and CO 3 2- In order to add carbon source, the same batch of Lanyue Lake seawater was used as the medium and grouped and labeled, and Ca 2+ The most suitable concentration range is used as a group, and five bottles are set as a control at a time, and multiple concentration changes are carried out; Mg 2+ , Fe 3+ Experiment with NaCl in the same way, HCO 3 - and CO 3 2- The total amount of carbon source was added as a group, and five bottles were set as a control at a time, and the concentration test was carried out multiple times; after the five groups of experiments were found to find their respective optimum...

Embodiment 2

[0037] This implementation case demonstrates a kind of preparation method of novel Spirulina medium according to the following steps:

[0038] (1) get seawater first, measure the Ca in the seawater 2+ , Mg 2+ , Fe 3+ , NaCl, and carbon source concentration values;

[0039] (2) with Ca 2+ 0.4g / L, Mg 2+ 1.31g / L, Fe 3+ 0.20g / L, NaCl is 32‰, and carbon source is 0.17g / L as the reference value of the basic seawater, adjust the concentration value of the seawater according to the reference value, obtain the seawater reserve liquid, make the seawater reserve liquid Ca 2+ , Mg 2+ , Fe 3+ , NaCl, carbon source content and Ca in the basic seawater 2+ , Mg 2+ , Fe 3+ , NaCl, and carbon source content are the same;

[0040] (3) adjust the pH value of the seawater standby solution to 6.8, add Fe at a temperature of 35°C 3+ 0.003g / L, stir for 3min at a centrifugal speed of 3000r / min, pass through a 0.12μm filter membrane, adjust the pH value to 6.3, add 0.15g / L of carbon source...

Embodiment 3

[0043] This implementation case demonstrates a kind of preparation method of novel Spirulina medium according to the following steps:

[0044] (1) get seawater first, measure the Ca in the seawater 2+ , Mg 2+ , Fe 3+ , NaCl, and carbon source concentration values;

[0045] (2) with Ca 2+ 0.4g / L, Mg 2+ 1.31g / L, Fe 3+ 0.20g / L, NaCl is 32‰, and carbon source is 0.17g / L as the reference value of the basic seawater, adjust the concentration value of the seawater according to the reference value, obtain the seawater reserve liquid, make the seawater reserve liquid Ca 2+ , Mg 2+ , Fe 3+ , NaCl, carbon source content and Ca in the basic seawater 2+ , Mg 2+ , Fe 3+ , NaCl, and carbon source content are the same;

[0046] (3) adjust the pH value of the seawater standby solution to 7, add Fe at a temperature of 36°C 3+ 0.0035g / L, stir for 4min at a centrifugal speed of 3500r / min, pass through a 0.13μm filter membrane, adjust the pH value to 6.7, add carbon source 0.17g / L at ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a novel spirulina culture medium. The novel spirulina culture medium is prepared from 0.010g / L-0.015g / L of Ca<2+>, 0.015g / L-0.020g / L of Mg<2+>, 0.003g / L-0.004g / L of Fe<3+>, 1.5g / L of NaCl, 0.15g / L-0.18g / L of a carbon source and the balance basic seawater. Spirulina cultured by using the spirulina culture medium is high in yield and low in culture cost.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to a novel spirulina culture medium. Background technique [0002] In the early stage of industrial development of spirulina, the international community began to pay attention to the mariculture technology of spirulina. Because seawater is rich in various nutrient elements, seawater culture medium only needs to add four elements such as carbon, nitrogen, phosphorus, and iron to natural seawater to meet the growth needs of Spirulina, greatly reducing the amount of culture fertilizer and culture cost. The study found that Spirulina has good adaptability to high salinity and high concentration of calcium and magnesium ions in seawater, and maintains a good dominant species status in high salinity environment, reducing other biological pollution. The increasing shortage of fresh water resources and terrestrial arable land resources is also another main reason for...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 杨雯崔大练马玉心章敏陈晓丹
Owner ZHEJIANG OCEAN UNIV