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Method for promoting spore formation of bacillus coagulans

A technology of Bacillus coagulans and spores, which is applied in the field of biological fermentation, can solve the problems of unstable spore formation rate and product quality, complex medium components, and increased production costs, so as to shorten the product cycle, shorten the spore formation time, and improve production. rate effect

Active Publication Date: 2019-06-14
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there are many studies on high-density culture and spore-promoting culture of Bacillus coagulans at home and abroad in recent years, all of which are optimized from the medium and culture methods of Bacillus coagulans, but the research that can take into account high-density culture and improve the rate of spore formation rarely reported
At present, in the industrial production of Bacillus, there are generally problems such as spore formation rate, unstable product quality, and complex medium components. Generally, the temperature, dissolved oxygen, and stirring speed during the fermentation process are changed to increase the spore yield and shorten the fermentation time. The above method is cumbersome to operate in actual operation, consumes a lot of energy, and increases the production cost

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Embodiment 1

[0019] A method for promoting the formation of bacillus coagulans spores, the specific steps are as follows:

[0020] 1) Connect the original strain of Bacillus coagulans to the primary medium for activation, the inoculum volume ratio is 1:15, the filling volume is 30 mL / 250mL, the culture conditions are shaker speed 200rpm, culture temperature 36 °C, the culture time is 22 hours, and the first-grade strains are obtained after the culture is completed. The primary medium is composed of the following components according to mass percentage: glucose 1.8%, peptone 1.2%, yeast powder 0.6%, magnesium sulfate 0.3%, the balance is water; pH 7.8.

[0021] 2) Inoculate the primary strain of Bacillus coagulans activated in step 1) into the secondary liquid medium for expanded culture, the volume ratio of the inoculum volume is 1:30mL, and the liquid volume is 30 mL / 250mL; the culture conditions are The rotation speed of the shaker was 230 rpm / min, the culture temperature was 37° C., an...

Embodiment 2

[0026] A method for promoting bacillus coagulans spore formation, concrete steps:

[0027] 1) Connect the original strain of Bacillus coagulans to the primary medium for activation, the volume ratio of the inoculum volume is 1:25, the filling volume is 50 mL / 250 mL, the culture conditions are shaker speed 210 rpm / min, culture temperature The temperature is 37°C, the culture time is 20 hours, and the first-grade strains are obtained after the culture is completed. The primary medium is composed of the following components according to mass percentage: glucose 1.6%, peptone 1.4%, yeast powder 1.0%, magnesium sulfate 0.4%, the balance is water, pH 8.0.

[0028] 2) Inoculate the primary strain of Bacillus coagulans activated by step 1) into the secondary medium, the inoculum volume ratio is 1:25mL, and the liquid volume is 50mL / 250mL; the culture condition is that the rotation speed of the shaker is 220rpm / min, the culture temperature was 36.5°C, and the culture time was 18 hour...

Embodiment 3

[0033] A method for promoting the formation of bacillus coagulans spores, the specific steps are as follows:

[0034] 1) Connect the original strains to the primary medium for activation, the inoculum volume ratio is 1:25, the liquid volume is 50 mL / 250mL, the culture conditions are the shaker speed is 210rpm / min, and the culture temperature is 37.5°C , the cultivation time is 18 hours, and the first-grade strains are obtained after the cultivation is finished. The primary medium is composed of the following components according to mass percentage: glucose 1.7%, peptone 1.3%, yeast powder 0.9%, magnesium sulfate 0.5%, the balance is water, pH 8.2.

[0035] 2) Inoculate the primary strain of Bacillus coagulans activated by step 1) into the secondary medium, the volume ratio of the inoculum volume is 1:30mL, and the liquid volume is 30mL / 250mL; the culture condition is that the shaker speed is 230rpm / min, the culture temperature was 36.5°C, and the culture time was 16 hours. A...

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Abstract

The invention relates to a method for promoting spore formation of bacillus coagulans, and belongs to the technical field of biological fermentation. The method includes feeding a glucose solution andcalcium carbonate sequentially at intervals to a medium in the initial stage of the logarithmic growth of bacillus coagulans fermentation culture to promote the spore formation. Compared with the prior art, the method achieves the effect that the supplement of a carbon source can promote the increase of the viable count, increase the thallus density and accelerate the spore formation. The addition of the calcium carbonate can effectively shorten the spore formation time and improve the tolerance of the spores while increasing the number of spores.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for promoting the formation of bacillus coagulans spores. Background technique [0002] Bacillus coagulans is a gram-positive bacterium (G+). The bacteria can produce both lactic acid and dormant sporophytes with strong tolerance; therefore, it has the advantages of both lactic acid bacteria and bacillus. Bacillus coagulans can form a large number of metabolites during the growth process, such as coagulin (Coagulin), L-lactic acid and lactosporin. It has probiotic properties such as increasing the number of intestinal probiotics, treating diarrhea, enhancing immunity, improving irritable bowel syndrome, lowering cholesterol, and inhibiting the growth of pathogenic bacteria; and has broad-spectrum antibacterial properties, which can replace antibiotics and effectively inhibit aquatic and animal husbandry The reproduction of harmful bacteria i...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20C12R1/07
Inventor 吴影田晶晶古绍彬孙建瑞李长福周艳林周子吕
Owner HENAN UNIV OF SCI & TECH
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