Microbial agent and application of microbial agent to prevention and treatment of constipation

A technology of microbial inoculum and microbial strains is applied in the field of microorganisms to achieve the effects of good application compliance, inhibiting intestinal peristalsis and reducing symptoms of constipation

Inactive Publication Date: 2019-06-18
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has not been applied to probiotics for human use. Through a large number of tests, the present invention found a strain of Bacillus amyloliquefaciens, a strain of animal intestinal isolates that is very effective in preventing and treating constipation.

Method used

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  • Microbial agent and application of microbial agent to prevention and treatment of constipation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 Preparation of microbial agent of the present invention (Bacillus amyloliquefaciens bacterium liquid or powder)

[0022] 1. Take out the strain preservation tube, scratch the plate with LB solid medium for recovery, and incubate at 37°C for 48 hours. Pick a single colony under the plate and inoculate it into 50 ml of LB medium, and culture it in an incubator with shaking at 37°C for 24 hours. The seeds are inoculated with 5% inoculation amount into a 5L large triangle flask with a liquid volume of 2LLB medium, shaken and cultured at 37°C for 20-28 hours, and the concentration of the bacteria solution is tested. The viable bacteria content exceeds 2 billion / ml, and it is ready As a liquid strain of Bacillus amyloliquefaciens;

[0023] 2. Use the liquid Bacillus amyloliquefaciens cultured in step 1 as seeds, inoculate into 600L fermentation medium, the inoculation amount is 10%, stir at 120r / min, the air volume per minute liquid-gas ratio is 1:1, and cultiva...

Embodiment 2

[0026] Example 2 The safety test of the microbial agent of the present invention (Bacillus amyloliquefaciens)

[0027]The Bacillus amyloliquefaciens liquid that the embodiment of the present invention 1 produces is through the rat acute toxicity test in Shaoyang College, and the conclusion result shows that the Bacillus amyloliquefaciens liquid is harmful to rats (feeding the Bacillus amyloliquefaciens liquid 14 of the present invention by 0.6ml / 10g body weight) day) Effect: All rats survived, moved freely, had smooth hair, normal diet, no secretions in the respiratory tract, eyes and mouth, no symptoms of poisoning were found, and their body weight increased. Compared with the control group of the blank group, the body weight of the rats fed with the Bacillus amyloliquefaciens solution increased significantly (P<0.001). At the end of the 14-day observation period, the rats were dislocated and dissected, and no obvious abnormalities were found in the heart, liver, spleen, lung...

Embodiment 3

[0028] Example 3 Bacillus amyloliquefaciens prevents and treats constipation in mice

[0029] Establishment of mouse constipation model

[0030] After adapting to the feeding environment for one week, the mice were subcutaneously injected with 5 mg / kg loperamide hydrochloride (Loperamide hydrochloride) physiological saline suspension, once a day at 9:00 and 18:00, for 7 consecutive days, and 20 of them were selected as The normal group was given the same dose of normal saline. Select mice with dry feces, reduced number, and reduced weight as constipated mice.

[0031] Grouping and Dosage

[0032] Select 80 mice with successful modeling and randomly divide them into model groups (Constipation, whole intestine Bacillus licheniformis group (after separating and purifying the whole intestine strain produced by Shenyang First Pharmaceutical Co., Ltd. of Northeast Pharmaceutical Group Company, centrifuge after pure-bred fermentation, The fermentation medium is the same as the...

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Abstract

The invention discloses a microbial agent and application of the microbial agent to prevention and treatment of constipation, and particularly relates to a microbial agent prepared from Bacillus amyloliquefaciensYHSH-58 through fermentation and application of the microbial agent to prevention and treatment of constipation. The Bacillus amyloliquefaciensYHSH-58 is preserved in the China general microbiological culture collection center, No. 3, No. 1 yard, West Beichen Road, Chaoyang District, Beijing, the preservation number is CGMCC NO. 13664, and the preservation date is February 16, 2017. The application range of the Bacillus amyloliquefaciensYHSH-58 is expanded, the application value of the Bacillus amyloliquefaciensYHSH-58 is improved, and the better effect on prevention and treatmentof constipation is achieved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a microbial agent and its application in the prevention and treatment of constipation, in particular to a microbial agent produced by Bacillus amyloliquefaciens Bacillus amyloliquefaciens YHSH-58 Application of the prepared microbial agent in preventing and treating constipation. Background technique [0002] Constipation is the most common digestive system disease clinically, and its incidence is different in different regions and reports by scholars. Generally speaking, the west is higher than the east, women are higher than men, the elderly are higher than young people, and children are sick Also ubiquitous. According to investigations, the incidence rate of constipation in western crowds is about 6%-23%. my country has a vast territory, and the incidence rate survey results in various places are not the same: 6.07% of urban and rural residents of Beijing's permanent pop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/742A61P1/10C12R1/07
Inventor 蒋常德胡艳晖黄进
Owner FOSHAN YANHUI BIOTECH CO LTD
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