Specification for hydrogen treatment of reperfusion injury of dermal microvascular endothelial cells

Abstract

The invention discloses a manufacture method of a hydrogen saturated medium, comprising dissolving hydrogen in a serum-free ECM (extracellular matrix) medium for 2 hours under the pressure of 0.4 MPa,saturating the medium, depressurizing to allow hydrogen to be released, manufacturing O2 and CO2 saturated ECM media in the same way, and mixing in a ratio of (H2:O2:CO2=75%:20%:5%). The invention also discloses a specification for treating reperfusion injury of dermal microvascular endothelial cells by hydrogen saturated medium and hydrogen; in cell culture, the mixed medium is placed in a culture bottle, hydrogen concentration is detected immediately with a hydrogen measurer; a mixed gas having 75% of hydrogen, 20% of oxygen and 5% of carbon dioxide is introduced into a culture box; the concentrations of gases in the medium are kept balanced and stable; cells are added in the culture bottle and cultured in enclosed manner for 24 hours; the hydrogen content of the medium under the condition is about 0.6 MPa.

Description

technical field [0001] The invention mainly relates to the use of hydrogen gas in the cultivation of dermal microvascular endothelial cells in cell experiments, especially for alleviating the ischemia-reperfusion injury of dermal microvascular endothelial cells. Background technique [0002] In 2013, "Molecular Hydrogen Biology" edited by Sun Xuejun was published by the Second Military Medical University, which explained in detail the impact of hydrogen on human health. He pointed out that hydrogen has three properties, one is effectiveness: it has anti-oxidation effect, and the other is Safety: In addition to the anesthetic effect lower than that of nitrogen, hydrogen has almost no adverse effects. The third is economical: the effective dose is not particularly high, and hydrogen has a strong penetrability, which can easily penetrate the cell membrane and reach the cytoplasm, Cell organelles such as mitochondria. [0003] Ischemia-reperfusion refers to the main factor that...

AI Technical Summary

Problems solved by technology

Because ischemia-reperfusion injury can lead to damage and even apoptosis of dermal microvascular endothelial cells, but the use of drugs or other methods has little effect and often affects the normal metabolism of cells. Ischemia-reperfusion injury, but does not affect the normal metabolism of cells, thereby reducing the reperfusion injury of dermal microvascular endothelial cells

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