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Preparation method of nerve conduit material based on polyhydroxyalkanoate/gelatin electrospun nanofibers

A technology of polyhydroxyalkanoate and nerve conduit, which is applied in the field of preparation of nerve conduit materials, can solve the problems of insufficient donor area and limitation of autologous transplantation sources, and achieves the effects of improving biocompatibility and electrospinning performance.

Active Publication Date: 2020-08-04
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, autologous transplantation is the most commonly used clinical method for repairing peripheral nerve injuries, but autologous transplantation is limited by sources and insufficient donor sites.

Method used

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  • Preparation method of nerve conduit material based on polyhydroxyalkanoate/gelatin electrospun nanofibers
  • Preparation method of nerve conduit material based on polyhydroxyalkanoate/gelatin electrospun nanofibers
  • Preparation method of nerve conduit material based on polyhydroxyalkanoate/gelatin electrospun nanofibers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The nerve conduit material of polyhydroxyalkanoate / gelatin composite fiber is prepared according to the following method:

[0042] (1) At room temperature, weigh 0.17g of hyaluronic acid powder and 0.51g of chitosan, place chitosan in 50g of deionized water and put it in a low-temperature reactor at 4°C and stir evenly, then add dilute Add glacial acetic acid until the solution is clear, adjust the pH of the solution to 7.1 with β-sodium glycerophosphate, then add 5 mg of genipin and 0.17 g of hyaluronic acid and stir for 12 hours to prepare a CS-HA injection hydrogel solution for use;

[0043] (2) making the surface of the tubular mold (plastic tube) immersed in 70% sucrose bonding liquid with a concentration of 70% by mass, so that the bonding liquid is evenly distributed on the surface of the tubular mold;

[0044](3) Prepare a gelatin aqueous solution with a mass percentage concentration of 5%, extract 1 mL, add 0.4 g of alkyl glycosides and 32.34 g of chloroform, h...

Embodiment 2

[0052] The nerve conduit material of polyhydroxyalkanoate / gelatin composite fiber is prepared according to the following method:

[0053] (1) At room temperature, weigh 0.17g of hyaluronic acid powder and 0.51g of chitosan, place chitosan in 50g of deionized water and put it in a low-temperature reactor at 4°C and stir evenly, then add dilute Add glacial acetic acid until the solution is clear, adjust the pH of the solution to 7.1 with β-sodium glycerophosphate, then add 10 mg of genipin and 0.17 g of hyaluronic acid and stir for 12 hours to prepare a CS-HA injection hydrogel solution for use;

[0054] (2) making the surface of the tubular mold (plastic tube) immersed in a sucrose bonding solution with a mass percentage concentration of 60%, so that the bonding solution is evenly distributed on the surface of the tubular mold;

[0055] (3) Prepare a gelatin aqueous solution with a mass percentage concentration of 15%, extract 1 mL, add 0.4 g of alkyl glycosides and 32.34 g of ...

Embodiment 3

[0062] The nerve conduit material of polyhydroxyalkanoate / gelatin composite fiber is prepared according to the following method:

[0063] (1) At room temperature, weigh 0.17g of hyaluronic acid powder and 0.51g of chitosan, place chitosan in 50g of deionized water and put it in a low-temperature reactor at 4°C and stir evenly, then add dilute Add glacial acetic acid until the solution is clear, adjust the pH of the solution to 7.1 with β-sodium glycerophosphate, then add 5 mg of genipin and 0.17 g of hyaluronic acid and stir for 12 hours to prepare a CS-HA injection hydrogel solution for use;

[0064] (2) making the surface of the tubular mold (plastic tube) immersed in 70% sucrose bonding liquid with a concentration of 70% by mass, so that the bonding liquid is evenly distributed on the surface of the tubular mold;

[0065] (3) Prepare a gelatin aqueous solution with a concentration of 20% by mass, extract 1 mL, add 0.6 g of alkyl glycosides and 32.34 g of chloroform, homogen...

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Abstract

The invention discloses a preparing method of a nerve conduit material based on a polyhydroxyalkanoate / gelatin electrospun nanofiber. The nerve conduit material is obtained by winding a nanofiber on the surface of the nerve conduit base material, wherein the nanofiber is obtained from a spinning solution through electrostatic spinning. The spinning solution is a mixed solution of a gelatin water solution, alkyl glycoside, an organic solvent and polyhydroxyalkanoate. The nerve conduit material is obtained by coating the nanofiber layer on the surface of a tubular mold with hyaluronic acid / chitosan hydrogel. In the preparing method, alkyl glycoside is compounded with polyhydroxyalkanoate after wrapping the surface of gelatin to prepare a spinning solution, the electrospinning performance ofthe spinning solution is improved, processing is convenient, and good biocompability is realized.

Description

technical field [0001] The invention belongs to the technical field of preparation of medical composite fibers, and in particular relates to a preparation method of a nerve guide material based on polyhydroxyalkanoate / gelatin electrospun nanofibers. Background technique [0002] Human body trauma is inevitable in life, and the frequency of human body trauma in people's life is increasing year by year. Moreover, in human trauma, peripheral nerve damage accounts for about 5%. Therefore, nerve repair has become an important problem that needs to be solved urgently in the clinical and scientific circles. At present, autologous transplantation is the most commonly used clinical method to repair peripheral nerve injury, but autologous transplantation is limited by sources and insufficient donor sites. Thus, tissue engineered nerve conduits came into being. [0003] Polyhydroxyalkanoate (P(3HB-co-4HB)) is a degradable biopolyester obtained by microorganisms, and its biocompatibil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D04H1/4382D04H1/728D04H1/76D01D5/00D01D1/02A61L27/40A61L27/22A61L27/20A61L27/18A61L27/58
Inventor 郭静孙范忱刘赣东管福成
Owner DALIAN POLYTECHNIC UNIVERSITY
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