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Application of terpene compound in biological prevention and control of hyphantria cunea

A technology of American white moth and compounds, applied in pest control, chemicals for biological control, applications, etc., can solve problems such as lack of molecular mechanisms

Inactive Publication Date: 2019-07-05
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the function of OBPs in the insect olfactory response is still lack of specific molecular mechanism research

Method used

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  • Application of terpene compound in biological prevention and control of hyphantria cunea
  • Application of terpene compound in biological prevention and control of hyphantria cunea
  • Application of terpene compound in biological prevention and control of hyphantria cunea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The repelling effect of 3-carene on the American white moth

[0022] like figure 1 As shown, the application of the Y-type olfactometer experiment to detect the behavioral impact of 3-carene on the larvae of white moth larvae, the specific method includes the following steps:

[0023] The larvae of white moth were collected in the field and raised to the 5th instar with fresh poplar leaves.

[0024] Using liquid paraffin as a solvent, the 3-carene was prepared into five concentrations of 100mM, 50mM, 5mM, 0.5mM and 0.05mmol / L respectively.

[0025] Experiments were carried out with liquid paraffin as the blank control group. Use a micropipette to draw 1 μL of the drug, evenly drop it on a 2cm×0.5cm filter paper strip, put it into the sample tube, and then use another micropipette to draw 1 μL of liquid paraffin and evenly drop it on a 2cm×0.5cm filter paper strip. Put it on the filter paper strip and put it into another sample tube. Start the experiment after turni...

Embodiment 2

[0028] The Attractive Effect of 3-Carene on the Aphididae zhoui

[0029] like figure 2 As shown, the application of the Y-type olfactory instrument test 3-carene to the behavioral impact of the white moth Chow's gnats, the specific method includes the following steps:

[0030] The white moth Zhou's wasp cultured in the laboratory, the alternative host is tussah silkworm pupae.

[0031] Using liquid paraffin as a solvent, the 3-carene was prepared into five concentrations of 100mM, 50mM, 5mM, 0.5mM and 0.05mmol / L respectively.

[0032] Experiments were carried out with liquid paraffin as the blank control group. Use a micropipette to draw 1 μL of the drug, evenly drop it on a 2cm×0.5cm filter paper strip, put it into the sample tube, and then use another micropipette to draw 1 μL of liquid paraffin and evenly drop it on a 2cm×0.5cm filter paper strip. Put it on the filter paper strip and put it into another sample tube. Start the experiment after turning on the device swit...

Embodiment 3

[0035]Antennapotential Response to 3-carene of the white moth A. zhoui

[0036] like image 3 As shown, the EAG experiment is used to detect the antennal potential response of the white moth Choweri to 3-carene. The specific method includes the following steps:

[0037] Using liquid paraffin as a solvent, the 3-carene was prepared into five concentrations of 100mM, 50mM, 5mM, 0.5mM and 0.05mmol / L respectively.

[0038] Handling of the wasps: Dip the wasps that have emerged from the pupae into the finger-shaped test tube with a brush, take a wasp and place it under a dissecting microscope, cut off one antennae completely with a scalpel blade, and then cut off 1 / 2 of the wasp 3's torso and the tip of the other antennae.

[0039] Fixation of the wasp: apply an appropriate amount of conductive glue on the antennae and abdomen of the wasp, and fix the antennae and abdomen of the wasp treated with conductive glue on the two joints of the electrode respectively. Be careful not to s...

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Abstract

The invention discloses new use of a terpene compound (3-carene), and relates to application of the terpene compound in biological prevention and control of hyphantria cunea. It is proved through experiments that the 3-carene which is a main component of monoterpene in essential oil has a repellent effect on the hyphantria cunea and can be combined with olfactory binding proteins CcOBP2 to triggeran olfactory reaction so that natural enemy insects chouioia cunea Yang can be attracted. Through the application, research on the behavioral ecology of the hyphantria cunea and the natural enemies chouioia cunea Yang thereof plays an important role in the biological prevention and control of the hyphantria cunea, and the application prospects are broad.

Description

[0001] This invention was funded by the National Natural Science Foundation of China (31702058) and the general project of the Tianjin Applied Basic Program (18JCYBJC96300). technical field [0002] The invention relates to the application of a terpene compound in biological control of white moth, belonging to the technical fields of plant protection and pest control. Background technique [0003] American white moth, also known as autumn caterpillar, autumn caterpillar, and autumn curtain moth, is a worldwide quarantine pest with miscellaneous feeding habits, large reproduction, strong adaptability, wide transmission routes, and serious harm. It is listed as the first batch of "foreign pests" in my country. Invasive species" is listed by the State Forestry Administration as one of the six major project control pests. The State Forestry Administration issued the "Announcement on National White Moth Infested Areas in 2018 (No. 3 of 2018)", pointing out that the current nationa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N27/00A01P19/00A01P17/00A01G13/00
CPCA01G13/00A01N27/00
Inventor 潘丽娜李敏相伟芳杨艺新韩佳怡朱耿平
Owner TIANJIN NORMAL UNIVERSITY
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