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Application of sulfadiazine on the reproductive behavior of Trichogramma brevitus infected with wolbachia

A technology of sulfadiazine and Trichogramma, which is applied in the field of arthropod reproductive mode regulation, can solve problems such as the regulation mechanism that remains to be studied, achieve good application value and promotion significance, and eliminate the effect of influence

Active Publication Date: 2021-10-29
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The present Trichogramma genome and its in vivo sensing Wolbachia genomes have been published, but Wolbachia The regulation mechanism of Trichogramma parthenogenesis remains to be studied

Method used

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  • Application of sulfadiazine on the reproductive behavior of Trichogramma brevitus infected with wolbachia
  • Application of sulfadiazine on the reproductive behavior of Trichogramma brevitus infected with wolbachia
  • Application of sulfadiazine on the reproductive behavior of Trichogramma brevitus infected with wolbachia

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Embodiment 1

[0038] 1. Experimental method

[0039]Under the stereoscope, use a small writing brush to take out the short-tube Trichogramma females that have just emerged, put each head into the finger tube, and feed three kinds of antibiotic honey water respectively, and set up a control group. After feeding for 24 hours, put a lot of Parasitize on 100 fresh rice moth eggs that have been killed. After 24 hours of parasitism, use a small brush to pick out Trichogramma and put it in a centrifuge tube for storage at -30°C. Put the egg card in a finger tube and continue to put it in a climate box for cultivation. The temperature is 25°C, the relative humidity is 80% to 85%, and the light condition is L :D=14:10. Three sets of repetitions were performed for each treatment. Continuously treat 2 generations, record the number of eggs that turn black in each generation, that is, the number of eggs laid by Trichogramma, the number of females, males, and male and female mosaics, and calculate the...

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Abstract

The invention discloses the application of sulfadiazine to the reproductive behavior of Trichogramma brevis infected with Wolbachia. The invention provides the application of sulfadiazine in eliminating symbiotic Wolbachia or reducing the titer of Wolbachia in animals. The present invention finds for the first time that the honey water containing 1.0mg / ml sulfadiazine alone is used to feed the short-tube Trichogramma, which can well remove the Wolbachia carried by the short-tube Trichogramma. This method is more convenient, time-saving, and obtains complete male offspring. This will lay the foundation for the further use of Wolbachia to regulate the reproductive mode of arthropods, and has good application value and promotion significance.

Description

technical field [0001] The invention relates to the technical field of arthropod reproductive pattern regulation, more specifically, relates to the effect of sulfadiazine on infection Wolbachia Application of the short tube to the reproductive behavior of Trichogramma. Background technique [0002] Wolbachia It is a common intracellular symbiotic bacteria, and has a high infection rate in insects, mites and other arthropods and nematodes. The study shows that, based on the reported data, it is estimated by the model that 66% of the arthropods in nature are infected with Wolbachia . In the long-term evolution process, Wolbachia Possesses the ability to regulate the host's reproductive mode in the following ways: cause host cytoplasmic incompatibility, induce parthenogenesis, cause feminization of male genes, kill males, and enhance fecundity, so as to benefit their own survival and transmission, while affecting the host There are negative effects on reproduction. [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A61K31/635A61P31/04A01N43/54A01P1/00
CPCA01K67/033A01N43/54A61K31/635A61P31/04
Inventor 何晓芳张美娇王轩李远玉
Owner SOUTH CHINA AGRI UNIV
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