Aspartate aminotransferase mitochondrial isozyme detection kit and application

Abstract

The invention relates to an aspartate aminotransferase mitochondrion isozyme detection kit. The aspartate aminotransferase mitochondrion isozyme detection kit comprises the following components: the reagent 1 comprising 50 mmol / L of Tris buffer solution, 15 mmol / L of L-aspartic acid, 0.5 mmol / L of lactate dehydrogenase, more than 1 KU / L of the goat anti-human ASTs antibody, and more than 0.8 KU / Lof the malic dehydrogenase; the reagent 2 comprising 130 mmol / L of alpha-ketoglutaric acid and 130 mmol / L of NADH.

Description

technical field [0001] The invention relates to the field of immunodiagnosis, in particular to a detection kit for mitochondrial isozyme of aspartate aminotransferase and a use method thereof. Background technique [0002] Aspartate aminotransferase (AST) is widely present in various tissue cells of the human body, especially in liver cells and cardiomyocytes, so AST is often used in the detection of liver diseases and myocardial diseases. Electrophoretic analysis further divides AST into cytosolic AST (cytosolic AST, c-AST) and mitochondrial AST (mitochondrial AST, m-AST). The activity of AST in serum is the sum of the activities of c-AST and m-AST. AST, m-AST and c-AST are closely related. Currently, m-AST detection methods mainly include immunosuppressive method and enzymatic hydrolysis method, but the results of the two methods are different. [0003] This indicated that the specificity of m-AST, an immunosuppressive method, decreased when AST and m-AST increased, and...

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Problems solved by technology

It is also possible that the specificity of the anti-c-AST antibody is poor, and the active center of c-AST is not completely covered by the combination of the antibody and c-AST, resulting in that c-AST cannot be completely inhibited, and the m-AST measurement result is high