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Method for data analysis of miRNA in animals with reference data based on miRBase database

A data analysis and database technology, applied in genomics, bioinformatics, instrumentation, etc.

Active Publication Date: 2019-07-16
南京派森诺基因科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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There are also currently no existing tools for correlation analysis; or tools for linking these steps

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  • Method for data analysis of miRNA in animals with reference data based on miRBase database
  • Method for data analysis of miRNA in animals with reference data based on miRBase database
  • Method for data analysis of miRNA in animals with reference data based on miRBase database

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Embodiment Construction

[0041] The present invention will be further described by the following examples, but these examples should not be used to explain the limitation of the present invention.

[0042] see figure 1 , the detailed steps of the present invention include:

[0043] In step S1), the user's small RNA sequencing data and related database information are accepted, and then all data are analyzed to obtain annotation information of all small RNAs in each sample, and sequence characteristics analysis and expression of miRNAs are performed Quantitative analysis, as well as differential expression analysis between samples, function and pathway enrichment analysis.

[0044] The first is to filter and count the disembarkation data. In the embodiment of the present invention, the off-machine data is filtered to remove adapters and low-quality sequences, so as to obtain high-quality sequencing results. As an example, the perl language script is used to remove the linker sequence, and the origin...

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Abstract

The invention discloses a method for data analysis of miRNA in animals with reference data based on a miRBase database. The method is characterized by comprising the following steps of preparation, filtration of logout data, genome alignment, small RNA annotation, miRNA feature analysis, miRNA expression quantity analysis, miRNA function and pathway analyses, and result sorting. The method disclosed by the invention has the beneficial effects that an analysis result is complete, and comprises involved miRNA analysis content and other detected small RNA information annotation, automated analysis is achieved, and statistics and sorting of all analysis content and results can be automatically and rapidly conducted only by providing related database information. All operation steps are visible, and thus errors can be conveniently checked, applied command lines and parameters and log results generated in operation can all be recorded when analysis is conducted step by step, and once a program fails during running, the errors can be rapidly queried.

Description

technical field [0001] The invention relates to the field of transcriptome sequencing, in particular to a data analysis method for animal miRNA sequencing with reference data in a miRBase database. Background technique [0002] miRNA is a class of non-coding single-stranded RNA molecules encoded by endogenous genes, which participate in post-transcriptional gene expression regulation in animals and plants. Most miRNAs exist in the genome in the form of single copy, multiple copies or gene clusters. miRNAs are widely found in many species and are highly conserved in the evolutionary process. Therefore, studying the exact function of miRNAs, target genes, and their mechanisms of action is an important part of transcriptomics data analysis, which is crucial for understanding genes in organisms. The mechanism of expression regulation is of great significance. [0003] There are obvious differences in the mechanism of action of miRNA between animals and plants, and some species...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/20
CPCY02A90/10
Inventor 王玲平王智健姜丽荣沈立孙子奎
Owner 南京派森诺基因科技有限公司
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