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Method for assessing immunity and providing information on whether or not the onset of cancer has begun by utilizing difference in immune cell distribution between peripheral blood of colorectal cancer patient and normal person, and diagnostic kit using same

An immune cell, colorectal cancer technology, applied in disease diagnosis, particle and sedimentation analysis, analysis materials, etc., can solve problems such as difficulty in popularization, and achieve the effects of cost saving, high accuracy, and improved inspection reliability.

Active Publication Date: 2019-07-16
李宗均
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Problems solved by technology

For the prevention of cancer, a comprehensive and precise medical examination is very important, but the process takes time and cost, and considering the busy daily life of modern people, it is relatively difficult to popularize

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  • Method for assessing immunity and providing information on whether or not the onset of cancer has begun by utilizing difference in immune cell distribution between peripheral blood of colorectal cancer patient and normal person, and diagnostic kit using same
  • Method for assessing immunity and providing information on whether or not the onset of cancer has begun by utilizing difference in immune cell distribution between peripheral blood of colorectal cancer patient and normal person, and diagnostic kit using same
  • Method for assessing immunity and providing information on whether or not the onset of cancer has begun by utilizing difference in immune cell distribution between peripheral blood of colorectal cancer patient and normal person, and diagnostic kit using same

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[0025] The following detailed description of the present invention is a specific embodiment of the present invention, which will be described in conjunction with the accompanying drawings. The detailed description of the following examples is sufficient to enable those skilled in the art to practice the invention. It should be understood that, although the various embodiments of the invention may vary, they are not necessarily mutually exclusive. For example, a particular embodiment described herein may be embodied as another embodiment without departing from the spirit and principles of the invention. Alternatively, in the respectively described embodiments, the positions and sequences or arrangements of individual components, stages, and stages may be changed without departing from the spirit and principle of the present invention. Therefore, the following detailed description is not intended to limit the present invention, and if it can be properly explained, the scope of ...

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Abstract

Provided according to the present invention is a method for assessing immunity of peripheral blood and providing information on whether or not the onset of colorectal cancer has begun by utilizing a difference in immune cell distribution between peripheral blood of colorectal cancer patients and normal persons, the method comprising the steps of: (A) analyzing peripheral blood immune cells for cell size and wrinkle degree to classify the cells into lymphocytes, monocytes, and granulocytes; (B) staining a labeling marker of the three kinds of immune cells with at least one antibody combinationto analyze the distribution of immune cells in peripheral bloods of cancer patients and normal persons; (C) discriminating sets in which meaningful result values of the labeling marker are different between the two groups, cancer patients and normal persons, with statistical significance, so as to discriminate whether or not the onset of cancer has begun among cancer patients and normal persons; and (D) measuring immunity per unit of blood by means of the labeling marker to diagnose whether or not the onset of colorectal cancer has begun, without counting natural killer cells with a flow cytometer or a cell counter.

Description

technical field [0001] The present invention relates to a method of measuring and diagnosing the peripheral blood immunity of cancer patients including colorectal cancer and normal persons, finding markers showing a significant difference between the two groups, generating and applying markers based on the above Combined binary logistic regression algorithm to diagnose colorectal cancer patients and provide criteria for immunotherapy. Background technique [0002] Immunity is closely related to all processes such as the onset and metastasis of cancer. In many practical research literatures so far, although there are differences in degrees, it has been proposed that the immunity of most cancer patients is significantly reduced (Low) or damaged (Impaired) (Olivera J.Finn, Cancer Immunology, N Engl J Med, 2008:358:2704-15). Such research results have become an opportunity for people to break away from traditional cancer treatment methods (surgery and anticancer therapy) to ex...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/58G01N33/569G01N15/14
CPCG01N15/14G01N33/569G01N33/574G01N33/58G01N33/57496G01N33/582G01N33/56966G01N2015/1486G01N2800/7028G01N15/149
Inventor 李宗均崔重范
Owner 李宗均
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